| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HOXA1 |
| Uniprot No | P49639 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-335aa |
| 氨基酸序列 | MDNARMNSFL EYPILSSGDS GTCSARAYPS DHRITTFQSC AVSANSCGGD DRFLVGRGVQ IGSPHHHHHH HHHHPQPATY QTSGNLGVSY SHSSCGPSYG SQNFSAPYSP YALNQEADVS GGYPQCAPAV YSGNLSSPMV QHHHHHQGYA GGAVGSPQYI HHSYGQEHQS LALATYNNSL SPLHASHQEA CRSPASETSS PAQTFDWMKV KRNPPKTGKV GEYGYLGQPN AVRTNFTTKQ LTELEKEFHF NKYLTRARRV EIAASLQLNE TQVKIWFQNR RMKQKKREKE GLLPISPATP PGNDEKAEES SEKSSSSPCV PSPGSSTSDT LTTSH |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于HOXA1重组蛋白的3篇代表性文献(内容基于已有研究领域概括，部分信息为示例性整理)：
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1. **文献名称**: *"HOXA1 Recombinant Protein Regulates Early Hindbrain Patterning in Vertebrates"*
**作者**: Calvo, R., et al.
**摘要**: 研究利用重组HOXA1蛋白，揭示其在胚胎后脑发育中的调控作用，表明HOXA1通过激活特定靶基因(如PHOX2B)影响神经元分化与脑干区域化。
2. **文献名称**: *"Expression and Functional Characterization of Human HOXA1 in Breast Cancer Cells"*
**作者**: Zhang, Y., et al.
**摘要**: 通过体外表达重组HOXA1蛋白，发现其促进乳腺癌细胞侵袭和迁移，机制涉及EGFR/ERK信号通路的激活，提示HOXA1可能作为癌症治疗的潜在靶点。
3. **文献名称**: *"Purification and DNA-Binding Analysis of Recombinant HOXA1 Protein"*
**作者**: Smith, J., & Johnson, L.
**摘要**: 报道在大肠杆菌中高效表达并纯化HOXA1重组蛋白的方法，并验证其与特定DNA序列(如Hox响应元件)的结合能力，为后续功能研究提供工具。
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**备注**：上述文献标题与摘要为领域相关研究的概括性示例，具体文献需通过学术数据库(如PubMed、Web of Science)检索确认。
**Background of HOXA1 Recombinant Protein**
HOXA1. a member of the HOX family of transcription factors, plays a critical role in embryonic development, particularly in patterning the hindbrain, cranial neural crest cells, and cardiovascular structures. HOX genes encode DNA-binding proteins that regulate body segment identity and organogenesis by controlling the expression of downstream target genes. HOXA1. located on chromosome 7p15.2. is among the earliest HOX genes activated during embryogenesis, with its expression tightly regulated in time and space.
Dysregulation of HOXA1 has been linked to developmental disorders, such as Bosley-Salih-Alorainy syndrome, and cancers, including gliomas and breast cancer, where it may drive proliferation, invasion, or therapy resistance. Studying HOXA1's molecular mechanisms requires high-purity protein, prompting the development of recombinant HOXA1.
Recombinant HOXA1 protein is typically produced using bacterial (e.g., *E. coli*) or mammalian expression systems. These systems enable the incorporation of affinity tags (e.g., His or GST) for simplified purification. The protein retains functional domains, including the homeodomain responsible for DNA binding and protein-protein interactions.
Applications span *in vitro* assays (e.g., electrophoretic mobility shift assays to study DNA binding), cell-based studies to explore signaling pathways, and drug screening for HOXA1-related diseases. Its availability accelerates research into developmental biology, disease mechanisms, and therapeutic targeting. However, functional validation is essential to ensure bioactivity, as post-translational modifications may differ across expression systems.
In summary, HOXA1 recombinant protein serves as a vital tool for deciphering the gene's role in development and disease, bridging molecular insights to potential clinical applications.
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