| 纯度 | >92%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ILKAP |
| Uniprot No | Q9H0C8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-392aa |
| 氨基酸序列 | MDLFGDLPEP ERSPRPAAGK EAQKGPLLFD DLPPASSTDS GSGGPLLFDD LPPASSGDSG SLATSISQMV KTEGKGAKRK TSEEEKNGSE ELVEKKVCKA SSVIFGLKGY VAERKGEREE MQDAHVILND ITEECRPPSS LITRVSYFAV FDGHGGIRAS KFAAQNLHQN LIRKFPKGDV ISVEKTVKRC LLDTFKHTDE EFLKQASSQK PAWKDGSTAT CVLAVDNILY IANLGDSRAI LCRYNEESQK HAALSLSKEH NPTQYEERMR IQKAGGNVRD GRVLGVLEVS RSIGDGQYKR CGVTSVPDIR RCQLTPNDRF ILLACDGLFK VFTPEEAVNF ILSCLEDEKI QTREGKSAAD ARYEAACNRL ANKAVQRGSA DNVTVMVVRI GH |
| 预测分子量 | 44 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ILKAP重组蛋白的3篇参考文献的简要总结(注：文献为虚拟示例，实际研究中请核实具体文献)
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1. **文献名称**: *"Expression and functional characterization of recombinant ILKAP phosphatase in E. coli"*
**作者**: Lee, J. et al. (2016)
**摘要**: 该研究报道了在大肠杆菌中成功表达并纯化重组ILKAP蛋白，验证其磷酸酶活性，并发现其通过去磷酸化作用调控Akt信号通路，提示其在细胞凋亡中的潜在作用。
2. **文献名称**: *"ILKAP interacts with integrin-linked kinase (ILK) to modulate cell migration via MAPK signaling"*
**作者**: Miyake, T. et al. (2018)
**摘要**: 研究利用重组ILKAP蛋白进行体外结合实验，证实其与ILK的直接相互作用，并证明ILKAP通过负调控MAPK通路抑制肿瘤细胞迁移，为靶向治疗提供依据。
3. **文献名称**: *"Recombinant ILKAP induces apoptosis in glioma cells through caspase-3 activation"*
**作者**: Chen, X. et al. (2020)
**摘要**: 通过体外表达ILKAP重组蛋白，发现其可激活caspase-3依赖性凋亡通路，显著抑制胶质瘤细胞增殖，提示其在癌症治疗中的潜在应用价值。
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如需具体文献，建议在PubMed或Web of Science中以“ILKAP recombinant protein”为关键词检索最新研究。
ILKAP (Integrin-linked kinase-associated phosphatase), also known as protein phosphatase magnesium-dependent 1C (PPM1C), is a serine/threonine phosphatase belonging to the PPM family. It plays a regulatory role in cellular signaling pathways, particularly those involving integrin-mediated interactions and stress responses. ILKAP was initially identified as a binding partner of integrin-linked kinase (ILK), a key mediator of cell-matrix adhesion and survival signaling. By dephosphorylating ILK and other substrates, ILKAP modulates downstream pathways such as Akt/PKB and GSK-3β, influencing processes like apoptosis, proliferation, and migration.
Structurally, ILKAP contains a conserved phosphatase domain requiring Mg²⁺/Mn²⁺ ions for catalytic activity. Its function is context-dependent, acting as either a tumor suppressor or promoter in different cancers. For example, ILKAP downregulation has been linked to poor prognosis in hepatocellular carcinoma, while its overexpression enhances chemoresistance in leukemia. Beyond oncology, ILKAP regulates fibrosis by interacting with TGF-β/Smad pathways and affects cardiovascular health through endothelial cell signaling.
Recombinant ILKAP proteins, typically produced in *E. coli* or mammalian expression systems, retain enzymatic activity and are used to study phosphatase-substrate interactions, screen inhibitors, or explore structural mechanisms. Research focuses on its therapeutic potential—targeting ILKAP could modulate pathological signaling in cancer metastasis, fibrosis, or inflammation. However, its dual roles in cellular homeostasis and disease necessitate precise mechanistic studies. Current challenges include clarifying tissue-specific regulation and developing isoform-selective drugs, positioning ILKAP as a compelling yet complex target in biomedical research.
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