| 纯度 | ＞90% by SDS-PAGE. |
| 种属 | Human |
| 靶点 | YWHAE |
| Uniprot No | P62258 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-255aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMDDREDLVYQAKLAEQAERYDEMVESMKKV AGMDVELTVEERNLLSVAYKNVIGARRASWRIISSIEQKEENKGGEDKLK MIREYRQMVETELKLICCDILDVLDKHLIPAANTGESKVFYYKMKGDYHR YLAEFATGNDRKEAAENSLVAYKAASDIAMTELPPTHPIRLGLALNFSVF YYEILNSPDRACRLAKAAFDDAIAELDTLSEESYKDSTLIMQLLRDNLTL WTSDMQGDGEEQNKEALQDVEDENQ |
| 预测分子量 | 31 kDa |
| 蛋白标签 | C-his |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于YWHAE重组蛋白的示例参考文献(注：文献信息为示例性概括，非真实存在)：
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1. **《YWHAE重组蛋白的纯化及其在细胞信号通路中的作用》**
- 作者：Zhang L, et al.
- 摘要：研究通过原核表达系统(如大肠杆菌)成功纯化YWHAE重组蛋白，并验证其与MAPK通路中关键蛋白的相互作用，揭示了其在调控细胞增殖和凋亡中的功能。
2. **《14-3-3ε (YWHAE)重组蛋白的结构解析与磷酸肽结合特性》**
- 作者：Smith J, et al.
- 摘要：利用X射线晶体学解析了YWHAE重组蛋白的三维结构，阐明了其通过保守磷酸丝氨酸结合域与靶蛋白(如TP53)结合的分子机制。
3. **《YWHAE重组蛋白在神经退行性疾病模型中的应用》**
- 作者：Wang Y, et al.
- 摘要：体外实验表明，重组YWHAE蛋白可缓解tau蛋白异常聚集引起的神经元损伤，提示其在阿尔茨海默病治疗中的潜在价值。
4. **《高效表达YWHAE重组蛋白的哺乳动物细胞系统开发》**
- 作者：Lee H, et al.
- 摘要：优化了哺乳动物细胞表达体系，获得高纯度YWHAE重组蛋白，并证实其与癌症相关激酶(如PI3K)的功能性互作，为药物筛选提供工具。
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如需真实文献，建议通过PubMed或Google Scholar检索关键词“YWHAE recombinant protein”或“14-3-3 epsilon recombinant”，并筛选近5年研究。
YWHAE recombinant protein is a engineered form of the YWHAE (Tyrosine 3-Monooxygenase/Tryptophan 5-Monooxygenase Activation Protein Epsilon) protein, belonging to the highly conserved 14-3-3 protein family. These proteins function as adaptor molecules regulating diverse cellular processes, including signal transduction, cell cycle control, apoptosis, and metabolism. The YWHAE gene encodes a 30 kDa protein characterized by seven antiparallel α-helices forming a conserved phosphopeptide-binding groove. This structure enables interactions with phosphorylated serine/threonine motifs in client proteins, modulating their activity, stability, or subcellular localization.
Recombinant YWHAE is typically produced in bacterial (e.g., E. coli) or eukaryotic expression systems, ensuring proper post-translational modifications. Purification often involves affinity tags like GST or His-tag for enhanced solubility and yield. Its recombinant form retains the native ability to bind partners such as Raf kinases, CDC25 phosphatases, and apoptosis regulators like BAD, making it invaluable for studying phosphorylation-dependent interactions in vitro.
Research applications include investigating 14-3-3-mediated signaling pathways, validating protein-protein interactions via techniques like pull-down assays, and screening small molecules targeting 14-3-3/client interfaces. Dysregulation of YWHAE is linked to cancer, neurological disorders, and metabolic diseases, positioning the recombinant protein as a tool for mechanistic studies and therapeutic development. Recent studies also explore its chaperone-like activity in preventing protein aggregation, suggesting broader roles in cellular proteostasis.
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