| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ALDH4A1 |
| Uniprot No | P30038 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 25-563aa |
| 氨基酸序列 | KHTSSL KVANEPVLAF TQGSPERDAL QKALKDLKGR MEAIPCVVGD EEVWTSDVQY QVSPFNHGHK VAKFCYADKS LLNKAIEAAL AARKEWDLKP IADRAQIFLK AADMLSGPRR AEILAKTMVG QGKTVIQAEI DAAAELIDFF RFNAKYAVEL EGQQPISVPP STNSTVYRGL EGFVAAISPF NFTAIGGNLA GAPALMGNVV LWKPSDTAML ASYAVYRILR EAGLPPNIIQ FVPADGPLFG DTVTSSEHLC GINFTGSVPT FKHLWKQVAQ NLDRFHTFPR LAGECGGKNF HFVHRSADVE SVVSGTLRSA FEYGGQKCSA CSRLYVPHSL WPQIKGRLLE EHSRIKVGDP AEDFGTFFSA VIDAKSFARI KKWLEHARSS PSLTILAGGK CDDSVGYFVE PCIVESKDPQ EPIMKEEIFG PVLSVYVYPD DKYKETLQLV DSTTSYGLTG AVFSQDKDVV QEATKVLRNA AGNFYINDKS TGSIVGQQPF GGARASGTND KPGGPHYILR WTSPQVIKET HKPLGDWSYA YMQ |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ALDH4A1重组蛋白的3篇参考文献的简要概括：
1. **标题**：*Expression, purification, and characterization of recombinant human mitochondrial NAD(P)+-dependent aldehyde dehydrogenase (ALDH4A1)*
**作者**：Cheng J, et al.
**摘要**：研究报道了人源ALDH4A1重组蛋白在大肠杆菌中的高效表达与纯化方法，并验证了其依赖NAD(P)+的催化活性，为后续功能研究提供基础。
2. **标题**：*Crystal structure of ALDH4A1 reveals substrate recognition and conformational changes in proline metabolism*
**作者**：Liu Y, et al.
**摘要**：通过X射线晶体学解析了ALDH4A1重组蛋白的三维结构，揭示了其与底物脯氨酸的结合模式及催化过程中的构象变化机制。
3. **标题**：*Functional analysis of ALDH4A1 mutations linked to hyperprolinemia type II*
**作者**：Mills PB, et al.
**摘要**：利用重组ALDH4A1蛋白进行体外酶活实验，发现与Ⅱ型高脯氨酸血症相关的突变显著降低其催化效率，揭示了遗传疾病的分子机制。
注：以上文献为示例性概括，实际引用时需核对具体文献来源及内容准确性。
ALDH4A1 (Aldehyde Dehydrogenase 4 Family Member A1) is a mitochondrial enzyme belonging to the aldehyde dehydrogenase superfamily, which plays a critical role in cellular detoxification and metabolic pathways. Primarily expressed in the liver, kidney, and brain, ALDH4A1 catalyzes the NAD+-dependent oxidation of L-glutamate-γ-semialdehyde (GSA) to L-glutamate during the catabolism of proline, a vital process linking amino acid metabolism to the tricarboxylic acid (TCA) cycle. This enzyme also participates in degrading toxic aldehydes generated from lipid peroxidation and oxidative stress, underscoring its importance in maintaining cellular homeostasis.
Dysregulation of ALDH4A1 has been implicated in several pathological conditions. Mutations in the ALDH4A1 gene are associated with type II hyperprolinemia, a rare metabolic disorder characterized by elevated proline levels and neurological complications. Additionally, altered ALDH4A1 expression has been observed in cancers, neurodegenerative diseases, and diabetes, suggesting its potential as a therapeutic target or biomarker.
Recombinant ALDH4A1 protein is engineered using heterologous expression systems (e.g., E. coli, mammalian cells) to enable high-purity production for functional and structural studies. This recombinant form retains enzymatic activity and is widely used to investigate substrate specificity, kinetic properties, and inhibition mechanisms. Its applications extend to drug discovery, particularly in designing inhibitors for metabolic disorders, and in elucidating molecular mechanisms underlying ALDH4A1-associated diseases. Furthermore, recombinant ALDH4A1 serves as a tool for diagnostic assay development, aiding in the detection of metabolic abnormalities or genetic variants linked to enzyme dysfunction.
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