| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PRKD3 |
| Uniprot No | O94806 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-890aa |
| 氨基酸序列 | MSANNSPPSAQKSVLPTAIPAVLPAASPCSSPKTGLSARLSNGSFSAPSLTNSRGSVHTVSFLLQIGLTRESVTIEAQELSLSAVKDLVCSIVYQKFPECGFFGMYDKILLFRHDMNSENILQLITSADEIHEGDLVEVVLSALATVEDFQIRPHTLYVHSYKAPTFCDYCGEMLWGLVRQGLKCEGCGLNYHKRCAFKIPNNCSGVRKRRLSNVSLPGPGLSVPRPLQPEYVALPSEESHVHQEPSKRIPSWSGRPIWMEKMVMCRVKVPHTFAVHSYTRPTICQYCKRLLKGLFRQGMQCKDCKFNCHKRCASKVPRDCLGEVTFNGEPSSLGTDTDIPMDIDNNDINSDSSRGLDDTEEPSPPEDKMFFLDPSDLDVERDEEAVKTISPSTSNNIPLMRVVQSIKHTKRKSSTMVKEGWMVHYTSRDNLRKRHYWRLDSKCLTLFQNESGSKYYKEIPLSEILRISSPRDFTNISQGSNPHCFEIITDTMVYFVGENNGDSSHNPVLAATGVGLDVAQSWEKAIRQALMPVTPQASVCTSPGQGKDHKDLSTSISVSNCQIQENVDISTVYQIFADEVLGSGQFGIVYGGKHRKTGRDVAIKVIDKMRFPTKQESQLRNEVAILQNLHHPGIVNLECMFETPERVFVVMEKLHGDMLEMILSSEKSRLPERITKFMVTQILVALRNLHFKNIVHCDLKPENVLLASAEPFPQVKLCDFGFARIIGEKSFRRSVVGTPAYLAPEVLRSKGYNRSLDMWSVGVIIYVSLSGTFPFNEDEDINDQIQNAAFMYPPNPWREISGEAIDLINNLLQVKMRKRYSVDKSLSHPWLQDYQTWLDLREFETRIGERYITHESDDARWEIHAYTHNLVYPKHFIMAPNPDDMEEDP |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PRKD3重组蛋白的3篇示例参考文献(注：以下内容为模拟示例，非真实文献)：
1. **文献名称**：*"Recombinant Expression and Functional Characterization of Human PRKD3 in Cancer Cell Signaling"*
**作者**：Li, X., Zhang, Y., et al.
**摘要**：本研究通过大肠杆菌表达系统成功制备了重组人PRKD3蛋白，并验证其在体外激酶活性。实验表明，PRKD3通过磷酸化下游靶点STAT3促进肿瘤细胞侵袭，为癌症治疗提供潜在靶点。
2. **文献名称**：*"Structural Analysis of PRKD3 Kinase Domain via Recombinant Protein Crystallography"*
**作者**：Wang, H., Chen, L., et al.
**摘要**：利用杆状病毒-昆虫细胞系统表达并纯化PRKD3激酶结构域重组蛋白，通过X射线晶体学解析其三维结构，揭示了ATP结合口袋的特异性，为设计选择性抑制剂奠定基础。
3. **文献名称**：*"Optimization of PRKD3 Recombinant Protein Production in Mammalian Expression Systems"*
**作者**：Smith, J., Patel, R., et al.
**摘要**：对比HEK293和CHO细胞中PRKD3重组蛋白的表达效率，优化了培养条件和纯化方案，获得高活性、高纯度蛋白，适用于高通量药物筛选。
4. **文献名称**：*"PRKD3 Recombinant Protein as a Novel Biomarker in Autoimmune Diseases"*
**作者**：Kim, S., Tanaka, M., et al.
**摘要**：通过真核重组PRKD3蛋白检测患者血清中自身抗体水平，发现其与类风湿性关节炎的疾病活动度显著相关，提示PRKD3可能参与自身免疫调控机制。
(提示：实际研究中建议通过PubMed或Web of Science等数据库检索真实文献。)
PRKD3 (Protein Kinase D3), also known as PKCν, is a serine/threonine kinase belonging to the protein kinase D (PKD) family, which includes PRKD1. PRKD2. and PRKD3. It plays a critical role in intracellular signal transduction pathways regulating cell proliferation, apoptosis, migration, and immune responses. PRKD3 is activated by diacylglycerol (DAG) and protein kinase C (PKC) via phosphorylation, enabling its interaction with downstream effectors. Unlike PRKD1 and PRKD2. PRKD3 exhibits distinct tissue-specific expression patterns, with higher levels observed in the brain, pancreas, and hematopoietic cells. Its involvement in NF-κB signaling, Golgi organization, and secretory pathways highlights its diverse cellular functions.
Recombinant PRKD3 protein is engineered for in vitro studies to dissect its enzymatic activity, substrate specificity, and regulatory mechanisms. Produced using expression systems like E. coli or mammalian cells, the recombinant protein typically includes affinity tags (e.g., His-tag) for purification. Researchers utilize it to investigate PRKD3's role in diseases, particularly cancers (e.g., leukemia, breast cancer) and inflammatory disorders, where aberrant PRKD3 signaling may drive pathogenesis. It also serves as a tool for screening kinase inhibitors or modulators, aiding drug discovery efforts.
Despite progress, challenges remain in understanding PRKD3's isoform-specific functions and context-dependent regulation. Recombinant PRKD3 facilitates structural studies, phosphorylation mapping, and pathway validation, bridging gaps in mechanistic knowledge. Its application extends to developing targeted therapies, as PRKD3 overexpression or hyperactivity is linked to chemoresistance and metastasis in certain malignancies. However, off-target effects and incomplete pathway crosstalk characterization necessitate cautious interpretation of experimental data. Ongoing research aims to clarify PRKD3's therapeutic potential while addressing technical limitations in recombinant protein-based assays.
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