| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ACOX1 |
| Uniprot No | Q15067 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-660aa |
| 氨基酸序列 | MNPDLRRERD SASFNPELLT HILDGSPEKT RRRREIENMI LNDPDFQHED LNFLTRSQRY EVAVRKSAIM VKKMREFGIA DPDEIMWFKK LHLVNFVEPV GLNYSMFIPT LLNQGTTAQK EKWLLSSKGL QIIGTYAQTE MGHGTHLRGL ETTATYDPET QEFILNSPTV TSIKWWPGGL GKTSNHAIVL AQLITKGKCY GLHAFIVPIR EIGTHKPLPG ITVGDIGPKF GYDEIDNGYL KMDNHRIPRE NMLMKYAQVK PDGTYVKPLS NKLTYGTMVF VRSFLVGEAA RALSKACTIA IRYSAVRHQS EIKPGEPEPQ ILDFQTQQYK LFPLLATAYA FQFVGAYMKE TYHRINEGIG QGDLSELPEL HALTAGLKAF TSWTANTGIE ACRMACGGHG YSHCSGLPNI YVNFTPSCTF EGENTVMMLQ TARFLMKSYD QVHSGKLVCG MVSYLNDLPS QRIQPQQVAV WPTMVDINSP ESLTEAYKLR AARLVEIAAK NLQKEVIHRK SKEVAWNLTS VDLVRASEAH CHYVVVKLFS EKLLKIQDKA IQAVLRSLCL LYSLYGISQN AGDFLQGSIM TEPQITQVNQ RVKELLTLIR SDAVALVDAF DFQDVTLGSV LGRYDGNVYE NLFEWAKNSP LNKAEVHESY KHLKSLQSKL |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ACOX1重组蛋白的3篇参考文献及其简要概括：
1. **文献名称**：*"Recombinant human acyl-CoA oxidase 1: expression, purification, and kinetic characterization in peroxisomal fatty acid oxidation"*
**作者**：Mannaerts, G.P., Van Veldhoven, P.P., et al.
**摘要**：该研究报道了人源ACOX1重组蛋白在大肠杆菌中的高效表达及纯化方法，并分析了其酶动力学特性，证实其在过氧化物酶体脂肪酸β-氧化中的关键作用。
2. **文献名称**：*"Crystal structure of recombinant rat acyl-CoA oxidase 1 reveals insights into substrate binding and catalytic mechanism"*
**作者**：Huyghe, S., Casteels, M., et al.
**摘要**：通过X射线晶体学解析了大鼠ACOX1重组蛋白的三维结构，揭示了其底物结合口袋的构象变化，为理解催化机制和设计靶向药物提供了结构基础。
3. **文献名称**：*"Functional analysis of ACOX1 mutations linked to peroxisomal disorders using recombinant protein expression"*
**作者**：Ferdinandusse, S., Denis, S., et al.
**摘要**：研究通过体外表达携带疾病相关突变的ACOX1重组蛋白，发现部分突变导致酶活性显著降低，阐明了ACOX1缺陷引发过氧化物酶体代谢紊乱的分子机制。
**备注**：以上文献为示例性质，实际引用时建议通过PubMed或Web of Science等平台核对最新研究。若需具体文献信息，可进一步提供研究方向或年份要求。
**Background of ACOX1 Recombinant Protein**
Acyl-CoA oxidase 1 (ACOX1) is a peroxisomal enzyme critical for fatty acid β-oxidation, a metabolic pathway that breaks down very-long-chain fatty acids (VLCFAs) and branched-chain fatty acids into shorter molecules for energy production. ACOX1 catalyzes the first and rate-limiting step of this process, oxidizing fatty acyl-CoA substrates to trans-2-enoyl-CoA while producing hydrogen peroxide. It is predominantly expressed in the liver and kidney, playing a vital role in lipid homeostasis.
The ACOX1 gene, located on human chromosome 17q25.1. encodes two splice variants: ACOX1a (the primary isoform) and ACOX1b, which differ in substrate specificity. Mutations in ACOX1 are linked to peroxisomal disorders, such as ACOX1 deficiency, characterized by neurodevelopmental impairments and hepatic dysfunction. Studying ACOX1’s structure and function is essential for understanding lipid metabolism disorders and developing therapeutic strategies.
Recombinant ACOX1 protein is produced using heterologous expression systems (e.g., *E. coli* or mammalian cells*) to enable biochemical and structural studies. Its purification often involves affinity chromatography, yielding active enzyme for *in vitro* assays. Researchers utilize recombinant ACOX1 to investigate enzyme kinetics, substrate preferences, and interactions with regulatory proteins. Additionally, it serves as a tool for drug screening, particularly for compounds targeting peroxisomal disorders or metabolic syndromes.
Recent studies also explore ACOX1’s role beyond metabolism, including its potential involvement in oxidative stress and inflammation. The availability of recombinant ACOX1 has accelerated mechanistic insights into peroxisomal pathways and facilitated the development of cellular and animal models for disease research. This protein remains a focal point in metabolic and pharmaceutical research due to its central role in lipid processing and disease pathology.
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