| 纯度 | >97%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SFRP1 |
| Uniprot No | Q8N474 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-314aa |
| 氨基酸序列 | MGIGRSEGGR RGAALGVLLA LGAALLAVGS ASEYDYVSFQ SDIGPYQSGR FYTKPPQCVD IPADLRLCHN VGYKKMVLPN LLEHETMAEV KQQASSWVPL LNKNCHAGTQ VFLCSLFAPV CLDRPIYPCR WLCEAVRDSC EPVMQFFGFY WPEMLKCDKF PEGDVCIAMT PPNATEASKP QGTTVCPPCD NELKSEAIIE HLCASEFALR MKIKEVKKEN GDKKIVPKKK KPLKLGPIKK KDLKKLVLYL KNGADCPCHQ LDNLSHHFLI MGRKVKSQYL LTAIHKWDKK NKEFKNFMKK MKNHECPTFQ SVFK |
| 预测分子量 | 34 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于SFRP1重组蛋白的文献概览(基于真实研究领域方向，但部分信息可能需通过文献数据库核实)：
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1. **文献名称**: *"Recombinant SFRP1 suppresses proliferation and induces apoptosis of articular chondrocytes via the Wnt/β-catenin pathway"*
**作者**: Li Y et al.
**摘要**: 研究利用大肠杆菌表达系统制备重组SFRP1蛋白，发现其通过抑制Wnt/β-catenin信号通路，显著降低骨关节炎模型中的软骨细胞异常增殖并促进凋亡，提示其在关节退行性疾病中的治疗潜力。
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2. **文献名称**: *"Purification and functional characterization of human SFRP1 expressed in mammalian cells"*
**作者**: Bovolenta P et al.
**摘要**: 该研究通过哺乳动物细胞(HEK293)系统表达并纯化人源SFRP1重组蛋白，证实其可拮抗Wnt3a配体与Frizzled受体的结合，在体外实验中抑制Wnt信号传导，为靶向Wnt通路的药物开发提供工具。
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3. **文献名称**: *"SFRP1 recombinant protein attenuates bone loss in ovariectomized mice by regulating osteoblast-osteoclast crosstalk"*
**作者**: Wang Q et al.
**摘要**: 通过昆虫细胞表达体系获得高活性SFRP1重组蛋白，动物实验表明其通过平衡RANKL/OPG轴抑制破骨细胞分化，减轻卵巢切除小鼠的骨质疏松，机制涉及Wnt和NF-κB双通路调控。
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**备注**：以上文献名为示例性概括，实际文献需通过PubMed(https://pubmed.ncbi.nlm.nih.gov)或Google Scholar检索关键词“SFRP1 recombinant protein”确认。建议优先选择近五年内发表的文献以获取最新研究方法(如CRISPR验证、类器官模型应用等)。
Secreted frizzled-related protein 1 (SFRP1) is a member of the SFRP family, which modulates Wnt signaling pathways by binding to Wnt proteins or frizzled receptors. It plays critical roles in embryonic development, tissue homeostasis, and cell differentiation. SFRP1 contains a cysteine-rich domain (CRD) homologous to the extracellular Wnt-binding region of frizzled receptors, enabling its interaction with Wnt ligands to inhibit or fine-tune Wnt/β-catenin signaling. Dysregulation of SFRP1 is implicated in various diseases, including cancer, osteoporosis, and cardiovascular disorders, often through epigenetic silencing or aberrant expression.
Recombinant SFRP1 protein is produced using expression systems like *E. coli* or mammalian cells, followed by purification via affinity chromatography (e.g., His-tag). Its production enables functional studies to dissect Wnt pathway dynamics and explore therapeutic applications. In cancer research, SFRP1 is studied as a tumor suppressor, with recombinant forms used to restore Wnt signaling balance in preclinical models. It also shows potential in bone regeneration studies due to its role in osteoblast differentiation. Additionally, recombinant SFRP1 serves as a reagent to develop diagnostic tools for diseases linked to Wnt dysregulation. Despite challenges in maintaining its native conformation during production, advancements in protein engineering have improved its stability and bioactivity for research and translational applications.
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