| 纯度 | >88%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PDE2A |
| Uniprot No | O00408 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-941aa |
| 氨基酸序列 | GQACGHSILCRSQQYPAARPAEPRGQQVFLKPDEPPPPPQPCADSLQDAL LSLGSVIDISGLQRAVKEALSAVLPRVETVYTYLLDGESQLVCEDPPHEL PQEGKVREAIISQKRLGCNGLGFSDLPGKPLARLVAPLAPDTQVLVMPLA DKEAGAVAAVILVHCGQLSDNEEWSLQAVEKHTLVALRRVQVLQQRGPRE APRAVQNPPEGTAEDQKGGAAYTDRDRKILQLCGELYDLDASSLQLKVLQ YLQQETRASRCCLLLVSEDNLQLSCKVIGDKVLGEEVSFPLTGCLGQVVE DKKSIQLKDLTSEDVQQLQSMLGCELQAMLCVPVISRATDQVVALACAFN KLEGDLFTDEDEHVIQHCFHYTSTVLTSTLAFQKEQKLKCECQALLQVAK NLFTHLDDVSVLLQEIITEARNLSNAEICSVFLLDQNELVAKVFDGGVVD DESYEIRIPADQGIAGHVATTGQILNIPDAYAHPLFYRGVDDSTGFRTRN ILCFPIKNENQEVIGVAELVNKINGPWFSKFDEDLATAFSIYCGISIAHS LLYKKVNEAQYRSHLANEMMMYHMKVSDDEYTKLLHDGIQPVAAIDSNFA SFTYTPRSLPEDDTSMAILSMLQDMNFINNYKIDCPTLARFCLMVKKGYR DPPYHNWMHAFSVSHFCYLLYKNLELTNYLEDIEIFALFISCMCHDLDHR GTNNSFQVASKSVLAALYSSEGSVMERHHFAQAIAILNTHGCNIFDHFSR KDYQRMLDLMRDIILATDLAHHLRIFKDLQKMAEVGYDRNNKQHHRLLLC LLMTSCDLSDQTKGWKTTRKIAELIYKEFFSQGDLEKAMGNRPMEMMDRE KAYIPELQISFMEHIAMPIYKLLQDLFPKAAELYERVASNREHWTKVSHK FTIRGLPSNNSLDFLDEEYEVPDLDGTRAPINGCCSLDAE |
| 预测分子量 | 106 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3-4条关于PDE2A重组蛋白的参考文献及其摘要概括：
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1. **文献名称**: "Expression and Characterization of Recombinant Human PDE2A: A Role in Feedback Regulation of cGMP Signaling"
**作者**: Podzuweit et al.
**摘要**: 报道了人源PDE2A在大肠杆菌中的重组表达与纯化，分析了其环核苷酸水解活性，并探讨其在cGMP/cAMP信号通路中的负反馈调控作用。
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2. **文献名称**: "Structural Basis for the Activity of PDE2A Specific Inhibitors"
**作者**: Liu et al.
**摘要**: 通过重组PDE2A蛋白的晶体结构解析，揭示了其催化结构域与选择性抑制剂的结合机制，为靶向药物设计提供了结构基础。
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3. **文献名称**: "Functional Characterization of PDE2A Isoforms in Neuronal Cells Using Recombinant Proteins"
**作者**: Martinez et al.
**摘要**: 利用昆虫细胞系统表达PDE2A两种剪接变体重组蛋白，比较了二者在神经元中的亚细胞定位及酶动力学差异，提示其功能特异性。
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4. **文献名称**: "High-Throughput Screening Assay Development for PDE2A Using a Recombinant Catalytic Domain"
**作者**: Thompson & Zhang
**摘要**: 开发了基于重组PDE2A催化结构域的高通量筛选平台，用于快速评估小分子抑制剂效能，推动神经退行性疾病药物研发。
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注：以上文献信息为模拟示例，实际文献需通过PubMed或Web of Science等数据库检索确认。
**Background of PDE2A Recombinant Protein**
Phosphodiesterase 2A (PDE2A) is a member of the cyclic nucleotide phosphodiesterase superfamily, which hydrolyzes the secondary messengers cyclic adenosine monophosphate (cAMP) and cyclic guanosine monophosphate (cGMP) to regulate cellular signaling pathways. PDE2A is uniquely characterized by its dual substrate specificity and activation by cGMP via its regulatory GAF-B domain, enabling cross-talk between cAMP and cGMP signaling. It is broadly expressed in tissues, including the brain, heart, and adrenal glands, and plays critical roles in processes such as ion channel regulation, cell proliferation, and neuronal signaling.
Recombinant PDE2A protein is produced using genetic engineering techniques, often in *E. coli* or mammalian expression systems (e.g., HEK293 cells), to ensure proper folding and post-translational modifications. The purified protein retains enzymatic activity and structural integrity, making it a vital tool for *in vitro* studies. Researchers employ PDE2A recombinant protein to investigate its kinetic properties, inhibitor screening for drug development, and mechanisms underlying diseases linked to PDE2A dysfunction, such as neurodegenerative disorders, cardiovascular diseases, and cancer.
Structural studies using recombinant PDE2A have revealed insights into its catalytic domain organization and allosteric regulation, aiding the design of selective inhibitors. Its role in cognitive function and pathological conditions has spurred interest in targeting PDE2A for therapeutic interventions, particularly in enhancing memory or treating mood disorders. The availability of recombinant PDE2A continues to advance both basic research and translational applications in cyclic nucleotide signaling.
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