| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NET1 |
| Uniprot No | Q7Z628 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-542aa |
| 氨基酸序列 | MVAHDETGGLLPIKRTIRVLDVNNQSFREQEEPSNKRVRPLARVTSLANLISPVRNGAVRRFGQTIQSFTLRGDHRSPASAQKFSSRSTVPTPAKRRSSALWSEMLDITMKESLTTREIRRQEAIYEMSRGEQDLIEDLKLARKAYHDPMLKLSIMSEEELTHIFGDLDSYIPLHEDLLTRIGEATKPDGTVEQIGHILVSWLPRLNAYRGYCSNQLAAKALLDQKKQDPRVQDFLQRCLESPFSRKLDLWSFLDIPRSRLVKYPLLLKEILKHTPKEHPDVQLLEDAILIIQGVLSDINLKKGESECQYYIDKLEYLDEKQRDPRIEASKVLLCHGELRSKSGHKLYIFLFQDILVLTRPVTRNERHSYQVYRQPIPVQELVLEDLQDGDVRMGGSFRGAFSNSEKAKNIFRIRFHDPSPAQSHTLQANDVFHKQQWFNCIRAAIAPFQSAGSPPELQGLPELHEECEGNHPSARKLTAQRRASTVSSVTQVEVDENAYRCGSGMQMAEDSKSLKTHQTQPGIRRARDKALSGGKRKETLV |
| 预测分子量 | 77.9kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇与NET1重组蛋白相关的虚构参考文献示例(基于领域常见研究方向概括)：
1. **文献名称**：*"Recombinant NET1 Protein Enhances RhoA Activation in Cancer Cell Motility"*
**作者**：Smith, J. et al. (2019)
**摘要**：研究报道了在大肠杆菌中成功表达并纯化重组人源NET1蛋白，验证其体外激活RhoA GTP酶的能力，并通过细胞实验证明其促进乳腺癌细胞迁移和侵袭的分子机制。
2. **文献名称**：*"Structural Insights into NET1-RhoA Interaction via Recombinant Protein Crystallography"*
**作者**：Zhang, L. et al. (2020)
**摘要**：通过重组表达NET1蛋白的DH-PH结构域，解析其与RhoA结合的晶体结构，揭示了关键氨基酸残基对信号转导的调控作用，为靶向NET1的药物设计提供结构基础。
3. **文献名称**：*"Development of a NET1 Recombinant Protein-Based Assay for High-Throughput Inhibitor Screening"*
**作者**：Chen, Y. et al. (2022)
**摘要**：利用重组NET1蛋白建立体外GEF活性检测体系，筛选出小分子抑制剂并验证其在抑制胃癌细胞增殖中的潜在治疗价值。
**注**：以上内容为模拟概括，实际文献需通过PubMed/Google Scholar等平台检索关键词(如“NET1 recombinant protein”“RhoGEF NET1”)获取。
**Background of NET1 Recombinant Protein**
NET1 (Neuroepithelial Cell Transforming Gene 1) is a member of the guanine nucleotide exchange factor (GEF) family, primarily known for activating RhoA GTPases, which regulate cytoskeletal dynamics, cell migration, and proliferation. Initially identified for its oncogenic potential in neuroepithelial cells, NET1 is implicated in cancer progression, particularly in gastric, breast, and cervical cancers, where its overexpression correlates with enhanced invasiveness and metastasis.
Structurally, NET1 contains conserved DH (Dbl homology) and PH (Pleckstrin homology) domains critical for its GEF activity. The DH domain facilitates RhoA activation by promoting GTP binding, while the PH domain aids in membrane localization or protein interactions. Alternative splicing generates two isoforms: NET1A (nuclear) and NET1B (cytoplasmic), differing in subcellular localization and regulatory roles.
Recombinant NET1 protein is engineered using expression systems like *E. coli* or mammalian cells, often tagged with His or GST for purification. This allows researchers to study its biochemical activity, including RhoA binding kinetics or GEF efficiency *in vitro*. It also serves as a tool for screening inhibitors targeting NET1-RhoA interactions in anticancer drug development.
In research, recombinant NET1 aids in elucidating mechanisms of EMT (epithelial-mesenchymal transition) and cytoskeletal remodeling. Its role in nuclear pathways, such as transcriptional regulation via β-catenin, further expands its relevance in developmental biology and disease. Overall, NET1 recombinant protein is vital for dissecting Rho GTPase signaling and exploring therapeutic strategies against NET1-driven pathologies.
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