| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PDE4B |
| Uniprot No | Q07343 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-736aa |
| 氨基酸序列 | MKKSRSVMTVMADDNVKDYFECSLSKSYSSSSNTLGIDLWRGRRCCSGNLQLPPLSQRQSERARTPEGDGISRPTTLPLTTLPSIAITTVSQECFDVENGPSPGRSPLDPQASSSAGLVLHATFPGHSQRRESFLYRSDSDYDLSPKAMSRNSSLPSEQHGDDLIVTPFAQVLASLRSVRNNFTILTNLHGTSNKRSPAASQPPVSRVNPQEESYQKLAMETLEELDWCLDQLETIQTYRSVSEMASNKFKRMLNRELTHLSEMSRSGNQVSEYISNTFLDKQNDVEIPSPTQKDREKKKKQQLMTQISGVKKLMHSSSLNNTSISRFGVNTENEDHLAKELEDLNKWGLNIFNVAGYSHNRPLTCIMYAIFQERDLLKTFRISSDTFITYMMTLEDHYHSDVAYHNSLHAADVAQSTHVLLSTPALDAVFTDLEILAAIFAAAIHDVDHPGVSNQFLINTNSELALMYNDESVLENHHLAVGFKLLQEEHCDIFMNLTKKQRQTLRKMVIDMVLATDMSKHMSLLADLKTMVETKKVTSSGVLLLDNYTDRIQVLRNMVHCADLSNPTKSLELYRQWTDRIMEEFFQQGDKERERGMEISPMCDKHTASVEKSQVGFIDYIVHPLWETWADLVQPDAQDILDTLEDNRNWYQSMIPQSPSPPLDEQNRDCQGLMEKFQFELTLDEEDSEGPEKEGEGHSYFSSTKTLCVIDPENRDSLGETDIDIATEDKSPVDT |
| 预测分子量 | 78 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PDE4B重组蛋白研究的3篇示例文献(注：以下内容为示例，非真实文献)：
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1. **文献名称**：*Expression and Purification of Recombinant PDE4B in Baculovirus-Insect Cell System*
**作者**：Smith A, et al.
**摘要**：本研究利用杆状病毒-昆虫细胞系统高效表达重组PDE4B蛋白，优化了纯化条件并获得高纯度蛋白。该蛋白具有水解cAMP的活性，为后续结构解析和药物筛选提供了可靠材料。
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2. **文献名称**：*Structural Basis of PDE4B Inhibition by Selective Antagonists*
**作者**：Johnson R, et al.
**摘要**：通过X射线晶体学解析了重组PDE4B与抑制剂复合物的三维结构，揭示了其催化域的关键结合位点，为开发靶向PDE4B的抗炎药物提供了结构基础。
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3. **文献名称**：*Functional Characterization of PDE4B Splice Variants in Neuronal Signaling*
**作者**：Lee H, et al.
**摘要**：构建了PDE4B不同剪接变体的重组蛋白，发现其亚细胞定位和cAMP水解效率存在显著差异，提示剪接变体在神经信号通路中的特异性调控作用。
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(如需真实文献，建议通过PubMed或Web of Science以“PDE4B recombinant”为关键词检索近年研究。)
**Background of PDE4B Recombinant Protein**
Phosphodiesterase 4B (PDE4B) is a member of the PDE4 enzyme family, which specifically hydrolyzes cyclic adenosine monophosphate (cAMP), a critical secondary messenger regulating cellular processes such as inflammation, immune response, and neuronal signaling. PDE4B plays a pivotal role in modulating cAMP levels, thereby influencing pathways linked to diseases like chronic obstructive pulmonary disease (COPD), asthma, depression, and schizophrenia. Its isoform-specific functions and tissue distribution make it a promising therapeutic target.
Recombinant PDE4B protein is engineered using heterologous expression systems (e.g., *E. coli*, insect, or mammalian cells*) to produce purified, functional enzyme for research and drug discovery. The protein typically retains key structural features, including catalytic domains responsible for cAMP hydrolysis and regulatory regions that influence substrate binding and inhibitor interactions.
Studies leveraging recombinant PDE4B aim to elucidate its structural biology, enzymatic kinetics, and interactions with inhibitors. For example, crystallographic analyses of recombinant PDE4B have revealed binding modes of selective inhibitors, guiding the design of therapeutics with reduced side effects compared to non-selective PDE4 blockers. Additionally, this protein is instrumental in high-throughput screening campaigns to identify novel modulators for inflammatory and neurological disorders.
The development of PDE4B recombinant protein also addresses challenges in isoform-specific targeting, as PDE4 subtypes (PDE4A, B, C, D) share high homology but differ in cellular roles. By enabling precise biochemical and pharmacological characterization, recombinant PDE4B advances the understanding of cAMP signaling dysregulation and fosters the development of targeted therapies, highlighting its importance in both basic research and translational medicine.
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