| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ENPP5 |
| Uniprot No | Q9UJA9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-429aa |
| 氨基酸序列 | MTSKFLLVSF ILAALSLSTT FSLQPDQQKV LLVSFDGFRW DYLYKVPTPH FHYIMKYGVH VKQVTNVFIT KTYPNHYTLV TGLFAENHGI VANDMFDPIR NKSFSLDHMN IYDSKFWEEA TPIWITNQRA GHTSGAAMWP GTDVKIHKRF PTHYMPYNES VSFEDRVAKI IEWFTSKEPI NLGLLYWEDP DDMGHHLGPD SPLMGPVISD IDKKLGYLIQ MLKKAKLWNT LNLIITSDHG MTQCSEERLI ELDQYLDKDH YTLIDQSPVA AILPKEGKFD EVYEALTHAH PNLTVYKKED VPERWHYKYN SRIQPIIAVA DEGWHILQNK SDDFLLGNHG YDNALADMHP IFLAHGPAFR KNFSKEAMNS TDLYPLLCHL LNITAMPHNG SFWNVQDLLN SAMPRVVPYT QSTILLPGSV KPAEYDQEG |
| 预测分子量 | 48 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ENPP5重组蛋白的参考文献示例(内容为虚构，仅作格式参考)：
1. **文献名称**: *"Recombinant ENPP5 Expression and Enzymatic Characterization in Insect Cells"*
**作者**: Tanaka, K. et al.
**摘要**: 研究利用杆状病毒-昆虫细胞系统表达重组ENPP5蛋白，纯化后证实其具有水解磷酸二酯键的活性，并对底物特异性进行了分析。
2. **文献名称**: *"ENPP5 Recombinant Protein Alleviates Pulmonary Fibrosis via Modulation of ATP Signaling"*
**作者**: Chen, L. et al.
**摘要**: 通过大肠杆菌表达重组ENPP5.发现其能降解细胞外ATP，在体外和小鼠模型中抑制成纤维细胞活化，减轻肺纤维化进程。
3. **文献名称**: *"Structural and Functional Insights into Human ENPP5 through Recombinant Protein Engineering"*
**作者**: Müller, R. et al.
**摘要**: 解析了重组ENPP5的晶体结构，揭示了其催化结构域的关键氨基酸残基，并通过突变实验验证了其在核苷酸代谢中的功能机制。
4. **文献名称**: *"Development of a Recombinant ENPP5-based Diagnostic Tool for Ovarian Cancer Biomarker Detection"*
**作者**: Gupta, S. et al.
**摘要**: 利用哺乳动物细胞表达的重组ENPP5蛋白开发新型ELISA检测方法，证明其在卵巢癌患者血清中特异性识别特定糖基化标志物。
(注：以上文献为示例，实际研究中请通过学术数据库检索真实文献。)
ENPP5 (Ectonucleotide Pyrophosphatase/Phosphodiesterase 5) is a member of the ENPP family, a group of enzymes involved in hydrolyzing extracellular nucleotides such as ATP, ADP, and pyrophosphate. These enzymes play critical roles in regulating purinergic signaling pathways, which influence diverse physiological processes, including inflammation, mineralization, and cellular communication. ENPP5. specifically, is characterized by its conserved catalytic domain but distinct substrate preferences compared to other ENPP members. It is primarily expressed in tissues like the brain, liver, and kidneys, though its full physiological role remains under investigation.
Recombinant ENPP5 protein is engineered using expression systems (e.g., mammalian cells, insect cells, or E. coli) to produce purified, bioactive enzyme for research and therapeutic applications. Its recombinant form enables precise study of enzymatic activity, substrate specificity, and interactions with inhibitors or modulators. Studies suggest ENPP5 may hydrolyze nucleotide sugars or cyclic nucleotides, potentially impacting glycosylation pathways or cyclic nucleotide signaling. Dysregulation of ENPP enzymes is linked to pathologies such as calcification disorders, insulin resistance, and cancer, making ENPP5 a target for drug development.
In research, recombinant ENPP5 aids in elucidating its structure-function relationships, developing assays for high-throughput screening, and exploring its role in disease models. Its applications extend to biomarker discovery and therapeutic antibody production. However, challenges include optimizing stability and activity in vitro. Overall, ENPP5 recombinant protein serves as a vital tool for advancing understanding of nucleotide metabolism and its implications in health and disease.
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