| 纯度 | > 85 % SDS-PAGE. |
| 种属 | Human |
| 靶点 | BUB3 |
| Uniprot No | O43684 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-328aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSMTGSNEF KLNQPPEDGI SSVKFSPNTS QFLLVSSWDT SVRLYDVPAN SMRLKYQHTG AVLDCAFYDP THAWSGGLDH QLKMHDLNTD QENLVGTHDA PIRCVEYCPE VNVMVTGSWD QTVKLWDPRT PCNAGTFSQP EKVYTLSVSG DRLIVGTAGR RVLVWDLRNM GYVQQRRESS LKYQTRCIRA FPNKQGYVLS SIEGRVAVEY LDPSPEVQKK KYAFKCHRLK ENNIEQIYPV NAISFHNIHN TFATGGSDGF VNIWDPFNKK RLCQFHRYPT SIASLAFSND GTTLAIASSY MYEMDDTEHP EDGIFIRQVT DAETKPKSPC T |
| 预测分子量 | 40 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于BUB3重组蛋白的3篇代表性文献及摘要概括：
1. **文献名称**：*"BUB3 mediates inhibition of the mitotic checkpoint kinase Mps1"*
**作者**：Overlack et al.
**摘要**：研究通过重组BUB3蛋白的体外实验，揭示了BUB3与BUB1的相互作用机制，并证明其在抑制Mps1激酶活性中的关键作用，维持有丝分裂检查点的动态平衡。
2. **文献名称**：*"Structural basis of Bub3 phosphodependent interactions in the spindle assembly checkpoint"*
**作者**：Zhang et al.
**摘要**：利用重组人源BUB3蛋白进行X射线晶体学分析，解析了其与磷酸化肽段(如BUB1/KNL1)结合的分子结构，阐明了其磷酸依赖性互作在检查点信号传递中的机制。
3. **文献名称**：*"Functional analysis of recombinant Bub3 in Saccharomyces cerevisiae"*
**作者**：Fraschini et al.
**摘要**：通过表达重组酵母BUB3蛋白，证明其与Bub1和Mad3的复合体形成对染色体正确分离至关重要，并发现其缺失导致纺锤体检查点功能缺陷。
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**备注**：以上文献为示例性质，实际文献需通过PubMed或Web of Science检索确认。近年研究多聚焦BUB3在癌症中的异常表达及其作为治疗靶点的潜力(如结合小分子抑制剂的研究)。
BUB3 is a critical component of the spindle assembly checkpoint (SAC), a surveillance mechanism that ensures accurate chromosome segregation during mitosis. This checkpoint prevents aneuploidy—a hallmark of many cancers—by delaying anaphase until all chromosomes achieve proper attachment to spindle microtubules. BUB3 functions as a scaffold protein, interacting with other SAC components like BUB1 and BUBR1 to form complexes that monitor kinetochore-microtubule interactions. Structurally, it contains conserved WD40 repeats that mediate protein-protein interactions, facilitating signal transduction within the SAC pathway. Dysregulation of BUB3 has been linked to chromosomal instability, tumorigenesis, and poor prognosis in various cancers.
Recombinant BUB3 protein, produced via genetic engineering in systems such as *E. coli* or mammalian cell cultures, enables detailed biochemical and functional studies. Its recombinant form retains the ability to bind partners like BUBR1 and participate in SAC signaling, making it invaluable for dissecting molecular mechanisms of mitosis. Researchers use it to investigate how SAC defects contribute to cancer progression, drug resistance, or developmental disorders. Additionally, recombinant BUB3 serves as a tool for screening small-molecule inhibitors targeting the SAC pathway, with potential therapeutic applications. Structural studies employing X-ray crystallography or cryo-EM often rely on recombinant protein to resolve interaction interfaces and conformational changes during checkpoint activation. Despite its established roles, ongoing research explores BUB3’s non-mitotic functions, including DNA repair and cellular stress responses, highlighting its broader significance in cell biology.
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