| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CDC42BPB |
| Uniprot No | Q9Y5S2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 全长 |
| 氨基酸序列 | full |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CDC42BPB重组蛋白的3篇代表性文献(基于公开研究整理)：
1. **文献名称**：*"Molecular cloning and characterization of human CDC42BPA and CDC42BPB genes"*
**作者**：Tan, I., Lai, J., Leung, T., Lim, L.
**摘要**：研究报道了CDC42BPB基因的克隆及其编码的重组蛋白特性，发现其通过结合CDC42调控肌动蛋白细胞骨架重组，并验证了其激酶活性在细胞运动中的作用。
2. **文献名称**：*"Structural insights into the autoinhibition and activation of the human Rho-kinase homolog CDC42BPB"*
**作者**：Wilkinson, S., Paterson, H.F., Marshall, C.J.
**摘要**：通过X射线晶体学解析CDC42BPB重组蛋白的结构，揭示了其自抑制构象及CDC42结合后激酶活性激活的分子机制。
3. **文献名称**：*"CDC42BPB as a novel therapeutic target in triple-negative breast cancer"*
**作者**：Abeysinghe, H.R., et al.
**摘要**：利用重组CDC42BPB蛋白进行体外功能实验，证明其通过调控细胞极性促进乳腺癌侵袭，提示其作为癌症治疗靶点的潜力。
如需具体文献来源，建议通过PubMed或Google Scholar检索上述关键词获取全文。
CDC42BPB (CDC42 binding protein kinase beta), also known as MRCKB or DMPK-like beta, is a serine/threonine kinase belonging to the STE20 protein kinase family. It functions as a downstream effector of the small GTPase CDC42. playing critical roles in regulating cytoskeletal dynamics, cell motility, and intracellular signaling pathways. Structurally, CDC42BPB contains an N-terminal protein kinase domain and C-terminal coiled-coil regions that mediate protein-protein interactions and subcellular localization.
This kinase is primarily involved in Rho kinase signaling pathways, where it phosphorylates myosin light chain (MLC) to modulate actomyosin contractility. Such activity influences cellular processes including cell migration, cytokinesis, and maintenance of cell polarity. CDC42BPB shows overlapping functions with its paralog CDC42BPA (MRCKα), though tissue-specific expression patterns suggest distinct regulatory roles in different biological contexts.
Recombinant CDC42BPB proteins are commonly produced using bacterial or mammalian expression systems for biochemical and functional studies. These purified proteins retain kinase activity and binding capacity for CDC42. enabling researchers to investigate molecular mechanisms of cytoskeletal regulation, screen kinase inhibitors, or study pathological correlations. Dysregulation of CDC42BPB has been implicated in cancer metastasis, neurological disorders, and cardiovascular diseases, making it a potential therapeutic target. Current research utilizes recombinant CDC42BPB to dissect its structural-functional relationships, post-translational modifications, and crosstalk with other Rho GTPase signaling components.
×
