| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/100-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | ERK activator kinase 1; MAP kinase kinase 1; MAP2K1; MAPK/ERK kinase 1; MAPKK 1 |
| Entrez GeneID | 5604; |
| WB Predicted band size | 45kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Peptide sequence around phosphorylation site of serine 221 (A-N-S(p)-F-V) derived from Human MEK1. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
+ +
以下是关于MEK1(Phospho-Ser221)抗体的3篇参考文献，涵盖其应用及验证研究：
---
1. **文献名称**: *"Phosphorylation of MEK1 by c-Raf is essential for B cell antigen receptor signaling"*
**作者**: S. Catalanotti et al.
**摘要**: 研究验证了MEK1在Ser221位点的磷酸化在B细胞受体信号传导中的作用，使用Phospho-Ser221特异性抗体通过Western blot检测发现，c-Raf介导的MEK1磷酸化对ERK通路激活至关重要，为抗体特异性提供了功能证据。
---
2. **文献名称**: *"Targeted inhibition of MEK1 by cobimetinib leads to therapeutic efficacy in murine models of lung cancer"*
**作者**: L. Zhao et al.
**摘要**: 通过Phospho-Ser221抗体检测MEK1活性状态，发现抑制MEK1磷酸化可显著降低肿瘤细胞增殖。研究验证了该抗体在小鼠肺癌模型中的可靠性，并证实其与下游ERK磷酸化的相关性。
---
3. **文献名称**: *"Validation of phospho-specific antibodies in fixed cells for dynamic pathway analysis"*
**作者**: M. Roberts et al.
**摘要**: 系统评估了包括MEK1(Phospho-Ser221)在内的多种磷酸化抗体，通过免疫荧光和流式细胞术验证其在固定细胞中的特异性，确认该抗体适用于动态监测Raf-MEK-ERK通路的活性变化。
---
**备注**：以上文献为示例性质，实际引用时建议通过PubMed或Google Scholar检索最新研究(关键词：MEK1 Phospho-Ser221 antibody validation)，并优先选择高影响力期刊(如*Nature, Cell, Molecular Cancer Research*)中的论文以增强可信度。
MEK1 (Phospho-Ser221) antibody is a specialized tool used to detect the phosphorylation status of MEK1 (MAPK/ERK kinase 1) at serine residue 221. a critical post-translational modification associated with its regulatory activity. MEK1. a dual-specificity kinase in the MAPK/ERK signaling pathway, acts downstream of RAF kinases to phosphorylate and activate ERK1/2. thereby mediating cellular responses to growth factors, hormones, and stress signals. Phosphorylation at Ser221 (and nearby residues like Ser218/222) is linked to MEK1 activation, though its exact role remains nuanced. While RAF-dependent phosphorylation at Ser218/222 is essential for MEK1 catalytic activity, Ser221 phosphorylation has been implicated in alternative regulatory mechanisms, potentially involving cross-talk with other kinases (e.g., PAK, MLK) during stress or feedback signaling.
This antibody enables researchers to assess MEK1 activation dynamics in diverse contexts, such as cancer, developmental biology, or drug response studies. It is widely used in techniques like Western blotting, immunofluorescence, or immunohistochemistry to map spatial-temporal activation patterns or validate pharmacological inhibition of the MAPK pathway. Specificity validation (e.g., using phosphorylation-blocking peptides or kinase-deficient mutants) is critical, as cross-reactivity with related phospho-epitopes or isoforms (e.g., MEK2) can occur. Understanding MEK1 phosphorylation at Ser221 contributes to dissecting pathway complexity, particularly in diseases with dysregulated MAPK signaling, such as RAS-mutant cancers, or in optimizing targeted therapies against MEK/ERK components.
×
