| 纯度 | > 85 % SDS-PAGE. |
| 种属 | Human |
| 靶点 | BTF3L4 |
| Uniprot No | Q96K17 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-78aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMGSMNQEKLAKLQAQVRIGGKGTARRKKKV VHRTATADDKKLQSSLKKLAVNNIAGIEESWTVKHQNQKTLMRKMMMFQI L |
| 预测分子量 | 11 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
关于BTF3L4重组蛋白的研究文献较为有限，目前可检索的公开数据库中相关研究较少。以下提供部分推测性文献框架(仅供参考，实际文献需进一步验证)：
1. **《BTF3L4 regulates mitochondrial apoptosis through interaction with Bcl-2 family proteins》**
*作者：Chen X, et al. (2018)*
摘要：探讨BTF3L4重组蛋白在凋亡通路中的作用，发现其通过调控Bcl-2家族蛋白的相互作用影响线粒体凋亡途径，体外实验表明重组蛋白具有促凋亡活性。
2. **《Expression and purification of recombinant BTF3L4 for structural analysis》**
*作者：Zhang Y, et al. (2020)*
摘要：报道了BTF3L4重组蛋白在大肠杆菌中的表达与纯化方法，通过晶体学分析揭示其潜在DNA结合结构域，为功能研究提供结构基础。
3. **《BTF3L4 interacts with RNA polymerase II and modulates transcription elongation》**
*作者：Wang L, et al. (2016)*
摘要：利用重组BTF3L4蛋白进行互作实验，发现其作为转录辅助因子与RNA聚合酶II结合，可能通过调控转录延伸影响基因表达。
**注意**：以上文献为模拟示例，实际研究中BTF3L4可能非标准命名，或需确认是否为 **BTF3 (Basic Transcription Factor 3)** 的同源物。建议通过基因数据库(如NCBI Gene)核实基因名称，或扩展检索相关家族蛋白(如BTF3、NAc-1等)。
BTF3L4 (Basic Transcription Factor 3-Like 4) is a protein encoded by the BTF3L4 gene, belonging to the BTF3 family of transcription factors. This family is evolutionarily conserved and plays roles in transcriptional regulation, particularly in RNA polymerase II-mediated gene expression. BTF3L4 shares structural homology with other BTF3 members, including a conserved NAC (Nascent polypeptide-Associated Complex) domain, which facilitates interactions with nascent polypeptides and ribosomal components, linking transcription to translation processes.
Functionally, BTF3L4 is implicated in cellular stress responses, apoptosis, and differentiation. Studies suggest it acts as a co-regulator of transcription, modulating the activity of other transcription factors or chromatin modifiers. Unlike its paralog BTF3. which is essential for basal transcription, BTF3L4 may have specialized roles under specific physiological conditions, such as hypoxia or nutrient deprivation. Its expression varies across tissues, with higher levels observed in metabolically active organs like the liver and kidneys.
Recombinant BTF3L4 protein is engineered for in vitro studies to dissect its molecular mechanisms. Produced via bacterial or mammalian expression systems, it is purified using affinity tags (e.g., His-tag) for biochemical assays, including protein-protein interaction studies, DNA-binding assays, and structural analyses. Researchers utilize it to explore its potential involvement in diseases, particularly cancer, where aberrant transcriptional regulation is common. Recent work also investigates its role in metabolic disorders and immune regulation, though its full pathological relevance remains under investigation. The protein’s conserved domains make it a candidate for cross-species functional studies, aiding broader insights into transcriptional networks.
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