| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NETO1 |
| Uniprot No | Q8TDF5-3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-344aa |
| 氨基酸序列 | MIHGRSVLHI VASLIILHLS GATKKGTEKQ TTSETQKSVQ CGTWTKHAEG GIFTSPNYPS KYPPDRECIY IIEAAPRQCI ELYFDEKYSI EPSWECKFDH IEVRDGPFGF SPIIGRFCGQ QNPPVIKSSG RFLWIKFFAD GELESMGFSA RYNFTPDPDF KDLGALKPLP ACEFEMGGSE GIVESIQIMK EGKATASEAV DCKWYIRAPP RSKIYLRFLD YEMQNSNECK RNFVAVYDGS SSVEDLKAKF CSTVANDVML RTGLGVIRMW ADEGSRNSRF QMLFTSFQEP PCEGNTFFCH SNMCINNTLV CNGLQNCVYP WDENHCKEKR KTSLLDQLTN TSGT |
| 预测分子量 | 38 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇与NETO1重组蛋白相关的文献摘要概括(注：文献信息为模拟示例，具体文献需通过学术数据库核实)：
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1. **文献名称**：*NETO1 is a novel auxiliary subunit of NMDA receptors*
**作者**：Ng, D. et al.
**摘要**：本研究首次报道NETO1作为NMDA受体的辅助亚基，通过重组蛋白表达技术证实NETO1与GluN2A亚基结合，增强NMDA受体在突触中的稳定性和功能，为研究神经突触可塑性提供新机制。
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2. **文献名称**：*Structural insights into NETO1-AMPA receptor interaction*
**作者**：Chen, S. et al.
**摘要**：利用重组NETO1蛋白进行X射线晶体学分析，揭示了NETO1的CUB结构域与AMPA受体亚基GluA1的结合模式，阐明其在调节AMPA受体门控动力学中的分子机制。
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3. **文献名称**：*NETO1 modulates kainate receptor signaling in hippocampal neurons*
**作者**：Wyeth, M.S. et al.
**摘要**：通过体外表达重组NETO1蛋白，结合电生理实验，证明NETO1通过调控红藻氨酸受体(KARs)的脱敏速率影响海马神经元的兴奋性突触传递，提示其在癫痫病理中的潜在作用。
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**提示**：建议通过PubMed或Google Scholar搜索关键词“NETO1 recombinant protein”“NETO1 auxiliary subunit”获取最新文献。
**Background of NETO1 Recombinant Protein**
NETO1 (Neuropilin and Tolloid-like protein 1) is an auxiliary subunit of ionotropic glutamate receptors, primarily known for modulating the function of N-methyl-D-aspartate (NMDA) and kainate receptors in the central nervous system. It is a single-pass transmembrane protein containing two extracellular CUB domains and one LDLa domain, which facilitate protein-protein interactions critical for synaptic plasticity, learning, and memory. NETO1 stabilizes receptor complexes at postsynaptic membranes, influencing receptor kinetics, ligand sensitivity, and trafficking, thereby fine-tuning excitatory neurotransmission.
Recombinant NETO1 protein is engineered using expression systems (e.g., mammalian, insect, or bacterial cells) to produce purified, functional NETO1 for research. This allows scientists to study its structural properties, receptor-binding mechanisms, and role in neurological pathways in vitro. The recombinant form often includes tags (e.g., His or Fc tags) for easier purification and detection.
Research highlights NETO1's involvement in neurodevelopmental and psychiatric disorders. Altered NETO1 expression is linked to conditions like schizophrenia, autism spectrum disorders, and epilepsy, suggesting its potential as a therapeutic target. Studies using recombinant NETO1 have elucidated its interactions with GluK2 (kainate receptor) and GluN2A (NMDA receptor), revealing how these complexes regulate synaptic strength and plasticity.
Furthermore, NETO1 recombinant proteins are tools for drug discovery, enabling high-throughput screening for compounds that modulate glutamate receptor activity. Such efforts aim to develop treatments for neurodegenerative diseases (e.g., Alzheimer’s) or cognitive impairments. Overall, NETO1 recombinant proteins bridge molecular insights with translational applications, advancing our understanding of synaptic biology and brain disorders.
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