| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SGK3 |
| Uniprot No | Q96BR1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-496aa |
| 氨基酸序列 | MQRDHTMDYK ESCPSVSIPS SDEHREKKKR FTVYKVLVSV GRSEWFVFRR YAEFDKLYNT LKKQFPAMAL KIPAKRIFGD NFDPDFIKQR RAGLNEFIQN LVRYPELYNH PDVRAFLQMD SPKHQSDPSE DEDERSSQKL HSTSQNINLG PSGNPHAKPT DFDFLKVIGK GSFGKVLLAK RKLDGKFYAV KVLQKKIVLN RKEQKHIMAE RNVLLKNVKH PFLVGLHYSF QTTEKLYFVL DFVNGGELFF HLQRERSFPE HRARFYAAEI ASALGYLHSI KIVYRDLKPE NILLDSVGHV VLTDFGLCKE GIAISDTTTT FCGTPEYLAP EVIRKQPYDN TVDWWCLGAV LYEMLYGLPP FYCRDVAEMY DNILHKPLSL RPGVSLTAWS ILEELLEKDR QNRLGAKEDF LEIQNHPFFE SLSWADLVQK KIPPPFNPNV AGPDDIRNFD TAFTEETVPY SVCVSSDYSI VNASVLEADD AFVGFSYAPP SEDLFL |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇与SGK3重组蛋白相关的参考文献，信息基于公开研究整理：
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1. **文献名称**：*"Expression and purification of active SGK3 kinase domain using a bacterial system"*
**作者**：Li X et al.
**摘要**：研究报道了利用大肠杆菌表达系统成功表达并纯化SGK3激酶结构域(aa 60-420)，通过Ni柱亲和层析获得高纯度蛋白。体外激酶实验证实重组SGK3具有磷酸化底物(如NDRG1)的活性，并揭示其活性受PI3K/mTOR信号通路的调控。
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2. **文献名称**：*"Structural insights into SGK3 regulation by phosphorylation and 14-3-3 binding"*
**作者**：Chen Y et al.
**摘要**：通过昆虫细胞表达系统制备全长人源SGK3重组蛋白，结合晶体学分析发现其C端Ser486磷酸化后与14-3-3蛋白结合，进而调控SGK3的亚细胞定位及激酶活性，为靶向SGK3的癌症治疗提供结构基础。
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3. **文献名称**：*"SGK3 promotes prostate cancer cell survival under androgen deprivation via mTORC2 activation"*
**作者**：Li H et al.
**摘要**：研究利用哺乳动物细胞(HEK293)表达重组SGK3.发现其通过磷酸化mTORC2复合物中的关键成分，在去势抵抗性前列腺癌中维持细胞存活，提示SGK3重组蛋白可作为药物筛选靶点。
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**注**：若需具体文献DOI或发表年份，建议在PubMed或Web of Science以“SGK3 recombinant”、“SGK3 kinase activity”为关键词检索近年研究。部分研究可能侧重SGK3在癌症或代谢疾病中的重组应用。
**Background of SGK3 Recombinant Protein**
Serum- and glucocorticoid-regulated kinase 3 (SGK3), a member of the serine/threonine kinase family, plays a critical role in cellular signaling pathways regulating cell survival, proliferation, and stress responses. It shares structural and functional similarities with SGK1 and SGK2 but exhibits distinct regulatory mechanisms and tissue expression patterns. SGK3 is primarily activated via the phosphoinositide 3-kinase (PI3K)/Akt/mTOR pathway, responding to growth factors, hormones (e.g., insulin), and cellular stressors. Its activation involves phosphorylation at specific residues, enabling interactions with downstream targets such as ion channels, transporters, and transcription factors.
SGK3 is implicated in diverse physiological processes, including electrolyte homeostasis, neuronal excitability, and autophagy. Dysregulation of SGK3 has been linked to pathological conditions such as cancer, neurodegenerative disorders, and metabolic diseases. For instance, SGK3 overexpression is observed in certain cancers (e.g., breast and prostate), where it promotes tumor cell survival under nutrient deprivation or hypoxia.
Recombinant SGK3 protein is engineered for in vitro studies to elucidate its molecular functions and therapeutic potential. Produced using heterologous expression systems (e.g., *E. coli*, mammalian cells), the recombinant protein retains kinase activity and structural integrity, enabling biochemical assays, drug screening, and interaction studies. Researchers utilize it to investigate SGK3’s role in signaling cascades, its crosstalk with other kinases (e.g., Akt), and its modulation by small molecules or genetic inhibitors.
Additionally, SGK3’s unique N-terminal phosphoinositide-binding domain (PHD) distinguishes it from other SGK isoforms, influencing its subcellular localization and activation. Recombinant SGK3 variants, including phosphorylation-mimetic or domain-deleted forms, are valuable tools for dissecting structure-function relationships and developing isoform-specific therapies. Overall, SGK3 recombinant protein serves as a pivotal resource for advancing understanding of cellular signaling and disease mechanisms.
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