| 纯度 | >95%SDS-PAGE. |
| 种属 | mouse |
| 靶点 | ENO2 |
| Uniprot No | P17183 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-434aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMGSMSIEKIWAREILDSRGNPTVEVDLYTA KGLFRAAVPSGASTGIYEALELRDGDKQRYLGKGVLKAVDHINSRIAPAL ISSGISVVEQEKLDNLMLELDGTENKSKFGANAILGVSLAVCKAGAAERD LPLYRHIAQLAGNSDLILPVPAFNVINGGSHAGNKLAMQEFMILPVGAES FRDAMRLGAEVYHTLKGVIKDKYGKDATNVGDEGGFAPNILENSEALELV KEAIDKAGYTEKMVIGMDVAASEFYRDGKYDLDFKSPADPSRYITGDQLG ALYQDFVRNYPVVSIEDPFDQDDWAAWSKFTANVGIQIVGDDLTVTNPKR IERAVEEKACNCLLLKVNQIGSVTEAIQACKLAQENGWGVMVSHRSGETE DTFIADLVVGLCTGQIKTGAPCRSERLAKYNQLMRIEEELGDEARFAGHN FRNPSVL |
| 预测分子量 | 50 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于ENO2重组蛋白的文献摘要信息(模拟示例，非真实文献)：
1. **文献名称**: "Recombinant Human ENO2 Protein Expression and Purification in Escherichia coli"
**作者**: Zhang L, et al.
**摘要**: 报道了利用大肠杆菌系统高效表达重组人ENO2蛋白的优化方法，通过亲和层析纯化获得高纯度蛋白，并验证其酶活性，为后续功能研究提供基础材料。
2. **文献名称**: "ENO2 as a Therapeutic Target in Glioblastoma: Recombinant ENO2 Antibody Inhibits Tumor Growth"
**作者**: Wang Y, et al.
**摘要**: 研究证实重组ENO2蛋白可制备特异性抗体，通过靶向抑制ENO2在胶质母细胞瘤中的表达，显著降低肿瘤细胞增殖能力，提示其作为癌症治疗靶点的潜力。
3. **文献名称**: "Autoantibody Detection Using Recombinant ENO2 in Autoimmune Encephalitis Diagnosis"
**作者**: Tanaka K, et al.
**摘要**: 开发基于重组ENO2蛋白的ELISA检测方法，用于识别自身免疫性脑炎患者血清中的抗ENO2抗体，提高诊断特异性，支持ENO2在神经系统自身免疫疾病中的标志物作用。
注：以上内容为示例，实际文献需通过PubMed/Google Scholar等平台以关键词"recombinant ENO2"或"ENO2 protein"检索。
**Background of ENO2 Recombinant Protein**
ENO2 (alpha-enolase 2), also known as neuron-specific enolase (NSE), is a glycolytic enzyme encoded by the *ENO2* gene. It catalyzes the dehydration of 2-phosphoglycerate to phosphoenolpyruvate during glycolysis, playing a critical role in cellular energy metabolism. ENO2 is predominantly expressed in neurons and neuroendocrine cells, distinguishing it from other enolase isoforms (ENO1 and ENO3) that are more widely distributed across tissues. Beyond its metabolic function, ENO2 exhibits "moonlighting" activities, participating in processes such as cell survival, hypoxia response, and immune regulation.
Recombinant ENO2 protein is engineered through molecular cloning, typically expressed in *E. coli* or mammalian systems, to ensure high purity and bioactivity. Its production enables detailed study of ENO2's structure-function relationships, interactions with binding partners (e.g., plasminogen), and roles in pathological conditions. Notably, ENO2 is a biomarker for neuroendocrine tumors, small cell lung cancer, and neuronal damage, as elevated levels correlate with disease progression.
Research also links ENO2 to neurodegenerative disorders, such as Alzheimer’s disease, where it interacts with pathological proteins like tau. Additionally, its overexpression in cancers promotes tumor growth, metastasis, and immune evasion, making it a potential therapeutic target. Recombinant ENO2 facilitates the development of diagnostic assays, antibody production, and inhibitor screening. However, challenges remain in understanding its tissue-specific regulation and non-glycolytic functions. Continued studies on recombinant ENO2 aim to unravel its multifaceted roles and translational applications in biomedicine.
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