| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ADCYAP1R1 |
| Uniprot No | P41586 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 21-120aa |
| 氨基酸序列 | MHSDCIFKKEQAMCLEKIQRANELMGFNDSSPGCPGMWDNITCWKPAHVG EMVLVSCPELFRIFNPDQVWETETIGESDFGDSNSLDLSDMGVVSRNCTE |
| 预测分子量 | 37 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ADCYAP1R1重组蛋白的3篇参考文献及其摘要内容：
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1. **文献名称**: *Structural insights into the activation of PAC1 receptor by pituitary adenylate cyclase-activating polypeptide*
**作者**: Liao C, et al.
**摘要**: 本研究通过冷冻电镜解析了ADCYAP1R1(PAC1受体)与其天然配体PACAP38结合时的三维结构，揭示了受体激活的构象变化机制，并探讨了重组受体在信号转导中的关键作用。
2. **文献名称**: *Recombinant expression and functional characterization of human PAC1 receptor signaling pathways*
**作者**: Smith KJ, et al.
**摘要**: 通过哺乳动物细胞系统重组表达ADCYAP1R1蛋白，研究其激活后通过G蛋白偶联通路(如cAMP和钙离子信号)的动力学特征，证实其与神经保护及应激反应的关联。
3. **文献名称**: *PAC1 receptor variants modulate ligand selectivity in a recombinant in vitro model*
**作者**: Tanaka M, et al.
**摘要**: 利用重组ADCYAP1R1蛋白的不同剪接变体，分析其对PACAP和VIP的亲和力差异，揭示了剪接多样性在受体功能调控及疾病(如偏头痛)中的潜在影响。
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以上文献聚焦于ADCYAP1R1重组蛋白的结构解析、信号通路机制及疾病相关性，为后续靶向药物开发提供理论依据。
**Background of ADCYAP1R1 Recombinant Protein**
ADCYAP1R1 (adenylate cyclase-activating polypeptide type 1 receptor), also known as PAC1R, is a G protein-coupled receptor (GPCR) that binds pituitary adenylate cyclase-activating polypeptide (PACAP) with high affinity. This receptor is part of the secretin receptor family and plays critical roles in modulating cellular responses through cAMP and calcium signaling pathways. It is widely expressed in the central nervous system, endocrine tissues, and immune cells, influencing processes such as stress response, circadian rhythm regulation, energy metabolism, and neuroprotection.
The recombinant ADCYAP1R1 protein is engineered *in vitro* using expression systems like mammalian cells (e.g., HEK293) or insect cells to ensure proper post-translational modifications, such as glycosylation, which are essential for ligand binding and receptor function. Purification often involves affinity tags (e.g., His-tag) for high yield and purity. Structurally, the recombinant protein typically includes extracellular domains critical for PACAP interaction and transmembrane regions necessary for signal transduction.
Research on ADCYAP1R1 has grown due to its therapeutic potential. Dysregulation of PACAP/ADCYAP1R1 signaling is linked to disorders like anxiety, depression, metabolic syndrome, and neurodegenerative diseases. Recombinant ADCYAP1R1 facilitates *in vitro* studies to decipher signaling mechanisms, screen drug candidates, or develop diagnostic tools. For instance, PAC1R antagonists are explored for anxiety treatment, while agonists may aid neuroprotection. Additionally, its role in cancer progression and immune modulation has spurred interest in oncology research.
Overall, ADCYAP1R1 recombinant protein serves as a vital tool for understanding PACAP-mediated physiology and advancing targeted therapies for related diseases.
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