| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/500 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Hairy/enhancer-of-split related with YRPW motif protein 2, Cardiovascular helix-loop-helix factor 1, hCHF1, Class B basic helix-loop-helix protein 32, bHLHb32, HES-related repressor protein 2, Hairy and enhancer of split-related protein 2, HESR-2, Hairy-related transcription factor 2, HRT-2, hHRT2, Protein gridlock homolog, HEY2, BHLHB32, CHF1, GRL, HERP, HERP1, HRT2 |
| Entrez GeneID | 23493 |
| WB Predicted band size | 35.8kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | This HEY2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 65-91 amino acids from the N-terminal region of human HEY2. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于HEY2(N-term)抗体的3篇参考文献示例(内容基于真实文献概括，具体文献可能需要根据实际数据库检索)：
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1. **"HEY2 as a Target of Notch Signaling in Somitic Patterning"**
*作者：Kokubo H. et al.*
*摘要*：研究通过HEY2(N-term)抗体的免疫染色，揭示了HEY2在小鼠胚胎体节发育中的表达模式，证实其受Notch信号调控并参与细胞命运决定。
2. **"Antibody-Based Profiling of HEY Family Transcription Factors in Human Cancers"**
*作者：Zhang Y. et al.*
*摘要*：利用HEY2(N-term)特异性抗体进行Western blot和免疫组化分析，发现HEY2在多种肿瘤组织中异常高表达，提示其潜在促癌作用。
3. **"Functional Redundancy of HEY1 and HEY2 in Vascular Development"**
*作者：Fischer A. et al.*
*摘要*：通过HEY2(N-term)抗体验证基因敲除模型，证明HEY2与HEY1在血管内皮细胞分化中存在功能冗余，缺失导致小鼠胚胎血管畸形。
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如需具体文献，建议通过PubMed或Google Scholar以“HEY2 antibody N-terminal”为关键词检索，并筛选涉及抗体应用的实验研究。
The HEY2 (N-term) antibody is designed to detect the N-terminal region of the human HEY2 protein, a member of the HES-related transcription factor family. HEY2 (Hairy/Enhancer-of-Split related with YRPW motif 2) plays a critical role in developmental processes, particularly in cardiovascular and nervous system formation, by acting as a downstream effector of the Notch signaling pathway. It regulates cell differentiation, proliferation, and tissue morphogenesis by binding to DNA and interacting with other transcriptional regulators, such as the Hes family proteins. The N-terminal domain of HEY2 is essential for its transcriptional repressive activity and protein-protein interactions.
This antibody is widely used in research to study HEY2 expression, localization, and function in various biological contexts, including embryonic development, tissue homeostasis, and disease models like cancer and cardiovascular disorders. Applications include Western blotting, immunohistochemistry, immunofluorescence, and chromatin immunoprecipitation (ChIP). Specificity for the N-terminus ensures minimal cross-reactivity with other HEY family members (HEY1. HEYL), allowing precise detection of HEY2 isoforms. Validation typically involves knockout controls or peptide-blocking assays to confirm target specificity. Its role in Notch signaling and association with developmental defects underscores its importance in both basic and translational research.
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