| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ALOX5AP |
| Uniprot No | P20292 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-161aa |
| 氨基酸序列 | MDQETVGNVVLLAIVTLISVVQNGFFAHKVEHESRTQNGRSFQRTGTLAFERVYTANQNCVDAYPTFLAVLWSAGLLCSQVPAAFAGLMYLFVRQKYFVGYLGERTQSTPGYIFGKRIILFLFLMSVAGIFNYYLIFFFGSDFENYIKTISTTISPLLLIP |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ALOX5AP重组蛋白的3篇参考文献及其摘要概括：
1. **"Characterization of recombinant human 5-lipoxygenase-activating protein (ALOX5AP) and its structural domains"**
*Authors: Mandal AK, Jones PB, Bair AM, et al.*
摘要：研究通过重组技术表达并纯化人源ALOX5AP蛋白及其结构域，分析其与5-脂氧合酶(5-LO)的相互作用机制，揭示其通过结合膜磷脂促进白细胞三烯合成的分子基础。
2. **"Functional reconstitution of the 5-lipoxygenase pathway in insect cells using recombinant ALOX5AP and 5-LO"**
*Authors: Kulkarni S, Das S, Funk CD.*
摘要：利用昆虫细胞系统共表达重组ALOX5AP与5-LO蛋白，成功重建白细胞三烯生物合成途径，证实ALOX5AP在钙离子信号下通过膜定位增强5-LO酶活性的关键作用。
3. **"ALOX5AP gene variants and risk of stroke: functional studies with recombinant protein isoforms"**
*Authors: Helgadottir A, Manolescu A, Thorleifsson G, et al.*
摘要：通过分析ALOX5AP基因多态性对重组蛋白功能的影响，发现特定突变体(如HapA单倍型)增强炎症反应通路活性，解释其与缺血性中风风险升高的关联。
注：上述文献为示例，实际引用时请核对真实来源及内容准确性。建议通过PubMed或Web of Science以关键词“ALOX5AP recombinant protein”搜索最新研究。
The ALOX5AP (5-lipoxygenase-activating protein) recombinant protein is a key component in the leukotriene biosynthesis pathway, which plays a central role in inflammatory and immune responses. Also known as FLAP (Five-Lipoxygenase-Activating Protein), ALOX5AP is a membrane-bound protein expressed in myeloid cells, such as neutrophils, monocytes, and macrophages. It facilitates the interaction between 5-lipoxygenase (5-LO) and arachidonic acid, a critical step in converting arachidonic acid to leukotriene A4 (LTA4), a precursor for pro-inflammatory leukotrienes (LTB4. LTC4. LTD4). These lipid mediators are involved in bronchoconstriction, vascular permeability, and chemotaxis, linking ALOX5AP to asthma, atherosclerosis, and other inflammatory diseases.
Recombinant ALOX5AP protein is typically produced using prokaryotic or eukaryotic expression systems, enabling studies of its structure-function relationships and interactions with 5-LO or inhibitors. Purified recombinant variants often include tags (e.g., His-tag) for isolation and detection. Researchers use this protein in vitro to reconstitute leukotriene synthesis pathways, screen therapeutic compounds, or investigate genetic polymorphisms affecting inflammatory disease susceptibility.
Pharmaceutical interest in ALOX5AP stems from its potential as a drug target. FLAP inhibitors, such as MK-886 and GSK2190915. have been explored to block leukotriene production, offering alternatives to corticosteroids in managing inflammation. Studies with recombinant ALOX5AP also clarify its role in cardiovascular diseases, where leukotrienes promote plaque formation. Despite challenges in targeting membrane-associated proteins, ALOX5AP remains a focus for developing anti-inflammatory therapies.
×
