| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TP63 |
| Uniprot No | Q9H3D4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-680aa |
| 氨基酸序列 | MNFETSRCATLQYCPDPYIQRFVETPAHFSWKESYYRSTMSQSTQTNEFLSPEVFQHIWDFLEQPICSVQPIDLNFVDEPSEDGATNKIEISMDCIRMQDSDLSDPMWPQYTNLGLLNSMDQQIQNGSSSTSPYNTDHAQNSVTAPSPYAQPSSTFDALSPSPAIPSNTDYPGPHSFDVSFQQSSTAKSATWTYSTELKKLYCQIAKTCPIQIKVMTPPPQGAVIRAMPVYKKAEHVTEVVKRCPNHELSREFNEGQIAPPSHLIRVEGNSHAQYVEDPITGRQSVLVPYEPPQVGTEFTTVLYNFMCNSSCVGGMNRRPILIIVTLETRDGQVLGRRCFEARICACPGRDRKADEDSIRKQQVSDSTKNGDGTKRPFRQNTHGIQMTSIKKRRSPDDELLYLPVRGRETYEMLLKIKESLELMQYLPQHTIETYRQQQQQQHQHLLQKQTSIQSPSSYGNSSPPLNKMNSMNKLPSVSQLINPQQRNALTPTTIPDGMGANIPMMGTHMPMAGDMNGLSPTQALPPPLSMPSTSHCTPPPPYPTDCSIVSFLARLGCSSCLDYFTTQGLTTIYQIEHYSMDDLASLKIPEQFRHAIWKGILDHRQLHEFSSPSHLLRTPSSASTVSVGSSETRGERVIDAVRFTLRQTISFPPRDEWNDFNFDMDARRNKQQRIKEEGE |
| 预测分子量 | 80.8kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于TP63重组蛋白的3篇代表性文献(基于公开研究整理)：
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1. **文献名称**：*TP63 isoforms regulate transcription of distinct gene sets to control cell fate and morphogenesis*
**作者**：Truong AB et al.
**摘要**：研究通过表达重组TP63蛋白的不同亚型(TAp63和ΔNp63)，结合ChIP-seq分析，揭示不同亚型通过结合特定DNA元件调控上皮发育和肿瘤发生的分子机制。
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2. **文献名称**：*Structural basis of DNA recognition by p63 and p73 homeodomain transcription factors*
**作者**：Ortega E et al.
**摘要**：利用重组TP63蛋白的DNA结合结构域进行X射线晶体学研究，阐明其与靶基因启动子结合的分子机制，揭示其与p53家族其他成员的结构差异。
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3. **文献名称**：*Recombinant p63 protein isoforms regulate keratinocyte differentiation through Notch and BMP signaling*
**作者**：Koster MI et al.
**摘要**：通过体外表达重组TP63蛋白，证明ΔNp63α亚型通过调控Notch和BMP通路维持表皮干细胞稳态，并抑制终末分化，为皮肤再生研究提供依据。
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如需获取具体文献全文，建议通过PubMed或Web of Science按标题检索DOI号，并访问期刊官网下载。
TP63 recombinant protein is derived from the TP63 gene, a member of the p53 tumor suppressor protein family. The TP63 gene encodes multiple isoforms through alternative splicing and promoter usage, primarily classified into two groups: TAp63 (transactivation domain-containing) and ΔNp63 (N-terminal truncated). These isoforms exhibit distinct functional roles, with TAp63 acting as a transcriptional activator involved in apoptosis and cell differentiation, while ΔNp63 often functions as a dominant-negative regulator, counteracting the activity of p53 and TAp63. TP63 plays critical roles in embryonic development, epithelial cell differentiation, and tissue homeostasis, particularly in skin, mammary glands, and prostate tissues.
Recombinant TP63 proteins are typically produced in bacterial or mammalian expression systems to study their structural and functional properties. These proteins retain key domains, including the DNA-binding domain, oligomerization domain, and C-terminal regulatory region. Researchers utilize purified TP63 recombinant proteins for in vitro assays, such as DNA-binding studies, protein-protein interaction analyses, and functional characterization of mutations linked to human diseases. For instance, mutations in TP63 are associated with developmental disorders like ectrodactyly-ectodermal dysplasia-clefting (EEC) syndrome and adult-onset cancers, particularly squamous cell carcinomas.
The recombinant form enables precise investigation of TP63’s dual role as both an oncogene and tumor suppressor, depending on isoform expression and cellular context. It also aids in exploring therapeutic strategies targeting TP63-mediated pathways in regenerative medicine and cancer treatment. Standardized production of TP63 recombinant proteins ensures reproducibility in studies addressing its complex regulatory networks and post-translational modifications, such as phosphorylation and ubiquitination, which modulate its stability and activity.
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