Recombinant Human IL4R protein

中文名： 白介素4受体(IL4R)重组蛋白
别名： IL4R；IL4RA；Interleukin-4 receptor subunit alpha

货号: PA1000-5406

Price： ￥询价

20μg 50μg 100μg ＞100μg

数量：

大包装询价

产品详情

纯度	>95%SDS-PAGE.
种属	Human
靶点	IL4R
Uniprot No	P24394
内毒素	< 0.01EU/μg
表达宿主	E.coli
表达区间	26-232aa
氨基酸序列	MKVLQEPTCVSDYMSISTCEWKMNGPTNCSTELRLLYQLVFLLSEAHTCI PENNGGAGCVCHLLMDDVVSADNYTLDLWAGQQLLWKGSFKPSEHVKPRA PGNLTVHTNVSDTLLLTWSNPYPPDNYLYNHLTYAVNIWSENDPADFRIY NVTYLEPSLRIAASTLKSGISYRARVRAWAQCYNTTWSEWSPSTKWHNSY REPFEQH
预测分子量	25 kDa
蛋白标签	His tag N-Terminus
缓冲液	PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
稳定性 & 储存条件	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
复溶	Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

参考文献

以下是关于IL4R重组蛋白的3篇参考文献及其摘要概括：

1. **"Structural basis of IL-4 receptor signaling modulation by therapeutic antibodies"**

- **作者**: Gandhi, N.A., Pirozzi, G., Graham, N.M.H.

- **摘要**: 该研究解析了IL4Rα亚基与其配体IL-4/IL-13结合的晶体结构，揭示了治疗性抗体(如Dupilumab)通过阻断受体二聚化抑制下游信号通路的分子机制，为自身免疫疾病治疗提供了结构基础。

2. **"Recombinant soluble IL-4 receptor inhibits interleukin-4-mediated responses in mice"**

- **作者**: Fanslow, W.C., et al.

- **摘要**: 研究通过重组可溶性IL4R(sIL-4R)在小鼠模型中验证其作为IL-4拮抗剂的功能，证明其能显著抑制Th2细胞分化和IgE产生，提示其在过敏性疾病中的治疗潜力。

3. **"IL-4 receptor α in human dendritic cells regulates T helper-2 responses and IgE production"**

- **作者**: Wills-Karp, M., et al.

- **摘要**: 利用重组IL4R蛋白研究树突状细胞中IL-4信号的作用，发现IL4Rα的激活促进Th2免疫应答，并揭示了其在哮喘患者中异常高表达的现象，为靶向IL4R的疗法提供了机制依据。

4. **"Engineering a high-affinity soluble mouse IL-4 receptor for therapeutic applications"**

- **作者**: Zhang, Y., et al.

- **摘要**: 报道了一种通过点突变增强重组小鼠IL4R结合亲和力的方法，实验显示改良后的受体蛋白在体内外模型中有效中和IL-4活性，为开发新型抗炎药物提供了技术路径。

这些文献涵盖了IL4R重组蛋白的结构解析、信号调控机制及治疗应用等方向。

背景信息

The interleukin-4 receptor (IL-4R) is a transmembrane protein critical in mediating immune responses, particularly in type 2 immunity. Composed of the IL-4Rα subunit paired with either the common gamma chain (γc, forming Type I IL-4R) or IL-13Rα1 (forming Type II IL-4R), it binds cytokines IL-4 and IL-13 to activate downstream signaling pathways like JAK-STAT, PI3K-AKT, and MAPK. These pathways regulate B-cell differentiation, IgE production, mucus secretion, and alternative macrophage activation, linking IL-4R to allergic inflammation, asthma, atopic dermatitis, and tumor microenvironment modulation.

Recombinant IL-4R proteins are engineered versions typically produced in mammalian expression systems (e.g., CHO or HEK293 cells) to ensure proper glycosylation and folding. They often contain extracellular domains necessary for ligand interaction, serving as decoy receptors or research tools to study receptor-ligand binding kinetics, competitive inhibition, and signaling crosstalk. In therapeutic contexts, IL-4R-targeting biologics like dupilumab (a monoclonal antibody blocking IL-4Rα) leverage this mechanism to treat Th2-mediated diseases, demonstrating the receptor's clinical relevance. Recombinant IL-4R variants are also used for structural studies, antibody drug development, and personalized medicine approaches in IL-4/IL-13-overexpressing cancers. Their standardized production enables consistent quality for both diagnostic assays and preclinical studies investigating immunoregulatory interventions.