| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | REG1B |
| Uniprot No | P48304 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-166aa |
| 氨基酸序列 | MAQTNSFFML ISSLMFLSLS QGQESQTELP NPRISCPEGT NAYRSYCYYF NEDPETWVDA DLYCQNMNSG NLVSVLTQAE GAFVASLIKE SSTDDSNVWI GLHDPKKNRR WHWSSGSLVS YKSWDTGSPS SANAGYCASL TSCSGFKKWK DESCEKKFSF VCKFKN |
| 预测分子量 | 18 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于REG1B重组蛋白的3篇参考文献及其简要摘要：
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1. **文献名称**: *Diagnostic value of serum REG1B as a biomarker for gastric cancer*
**作者**: Li Y, et al.
**摘要**: 该研究通过重组表达REG1B蛋白，验证其作为胃癌血清标志物的潜力，发现其在胃癌患者中显著高表达，且诊断敏感性和特异性优于传统标志物(如CEA)。
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2. **文献名称**: *Recombinant REG1B protein promotes pancreatic beta-cell regeneration in diabetic mice*
**作者**: Zhang H, et al.
**摘要**: 研究利用原核系统表达重组REG1B蛋白，并在糖尿病小鼠模型中证实其能促进胰岛β细胞再生，改善血糖控制，提示其在糖尿病治疗中的潜在应用。
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3. **文献名称**: *Prokaryotic expression and functional characterization of human REG1B in inflammation regulation*
**作者**: Wang J, et al.
**摘要**: 通过大肠杆菌系统高效表达可溶性REG1B重组蛋白，发现其能抑制巨噬细胞炎症因子释放，并激活ERK信号通路，为REG1B的免疫调节机制提供了实验依据。
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**备注**：以上文献为示例性质，具体内容需结合实际发表的论文调整。建议通过PubMed或Google Scholar以“REG1B recombinant protein”为关键词检索最新研究。
**Background of REG1B Recombinant Protein**
The REG1B (Regenerating Family Member 1B) protein, encoded by the *REG1B* gene, belongs to the REG family of secreted proteins involved in tissue regeneration and cellular proliferation. Initially identified for its role in pancreatic β-cell regeneration, REG1B is closely related to REG1A, sharing structural homology but differing in tissue-specific expression and regulatory mechanisms. Both proteins are characterized by a conserved calcium-dependent lectin domain and a functional secretory signal peptide, facilitating their extracellular roles.
REG1B is primarily expressed in the gastrointestinal tract, pancreas, and certain tumors. It functions as a mitogenic agent, promoting cell growth and survival, while also exhibiting anti-apoptotic and anti-inflammatory properties. Its overexpression is linked to pathological conditions, including diabetes, chronic inflammation, and cancers (e.g., gastric, colorectal), where it may drive tumor progression by enhancing cell proliferation and evading immune surveillance.
Recombinant REG1B is produced via genetic engineering in expression systems like *E. coli* or mammalian cells, ensuring high purity and bioactivity. This engineered protein retains native functional domains, enabling studies on its molecular interactions, signaling pathways (e.g., activation of MAPK/ERK or JAK/STAT pathways), and role in diseases. Researchers utilize REG1B recombinant proteins to investigate its potential as a diagnostic biomarker or therapeutic target, particularly in oncology. Challenges include elucidating its dual roles in regeneration versus tumorigenesis and optimizing delivery strategies for clinical applications. Current research focuses on deciphering its tissue-specific regulatory networks and exploring therapeutic interventions to modulate its activity in disease contexts.
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