| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PTGS2 |
| Uniprot No | P35354 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 18-601aa |
| 氨基酸序列 | ANPCCSHPCQNRGVCMSVGFDQYKCDCTRTGFYGENCSTPEFLTRIKLFLKPTPNTVHYILTHFKGFWNVVNNIPFLRNAIMSYVLTSRSHLIDSPPTYNADYGYKSWEAFSNLSYYTRALPPVPDDCPTPLGVKGKKQLPDSNEIVEKLLLRRKFIPDPQGSNMMFAFFAQHFTHQFFKTDHKRGPAFTNGLGHGVDLNHIYGETLARQRKLRLFKDGKMKYQIIDGEMYPPTVKDTQAEMIYPPQVPEHLRFAVGQEVFGLVPGLMMYATIWLREHNRVCDVLKQEHPEWGDEQLFQTSRLILIGETIKIVIEDYVQHLSGYHFKLKFDPELLFNKQFQYQNRIAAEFNTLYHWHPLLPDTFQIHDQKYNYQQFIYNNSILLEHGITQFVESFTRQIAGRVAGGRNVPPAVQKVSQASIDQSRQMKYQSFNEYRKRFMLKPYESFEELTGEKEMSAELEALYGDIDAVELYPALLVEKPRPDAIFGETMVEVGAPFSLKGLMGNVICSPAYWKPSTFGGEVGFQIINTASIQSLICNNVKGCPFTSFSVPDPELIKTVTINASSSRSGLDDINPTVLLKERS |
| 预测分子量 | 70.9kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于PTGS2(COX-2)重组蛋白研究的参考文献，按文献标题、作者和摘要内容概括整理：
1. **标题**：*Purification and characterization of recombinant human cyclooxygenase-2 (COX-2)*
**作者**：Smith WL, Garavito RM, DeWitt DL
**摘要**：该研究描述了在大肠杆菌中高效表达重组人COX-2蛋白的方法，通过亲和层析和离子交换层析纯化获得高纯度蛋白，并证实其具有与天然蛋白相似的酶活性和抑制剂敏感性。
2. **标题**：*Crystal structure of human cyclooxygenase-2 in complex with a selective inhibitor*
**作者**：Kurumbail RG, Stevens AM, Gierse JK, et al.
**摘要**：通过X射线晶体学解析了重组人COX-2蛋白与选择性抑制剂塞来昔布的复合物结构，揭示了抑制剂结合口袋的构象特征，为靶向COX-2的药物设计提供了结构基础。
3. **标题**：*Functional analysis of the catalytic activity of recombinant COX-2 in lipid mediator biosynthesis*
**作者**：Rowlinson SW, Kiefer JR, Prusakiewicz JJ, et al.
**摘要**：利用昆虫细胞表达系统制备重组COX-2蛋白，研究其催化花生四烯酸转化为前列腺素H2的动力学特性，并比较其与COX-1的底物选择性和抑制剂效应差异。
注：以上为示例性概括，具体文献内容需以原文为准。如需实际文献，建议通过PubMed或Web of Science检索关键词“PTGS2 recombinant protein”或“COX-2 purification”。
**Background of PTGS2 Recombinant Protein**
Prostaglandin-endoperoxide synthase 2 (PTGS2), also known as cyclooxygenase-2 (COX-2), is an inducible enzyme that catalyzes the conversion of arachidonic acid to prostaglandin H2. a precursor for prostanoids involved in inflammation, pain, fever, and cellular proliferation. Unlike its constitutive isoform PTGS1 (COX-1), PTGS2 is primarily expressed in response to inflammatory stimuli, growth factors, or oncogenic signals, making it a key therapeutic target for anti-inflammatory drugs (e.g., NSAIDs) and cancer therapies.
Recombinant PTGS2 protein is engineered using biotechnological platforms, such as bacterial, insect, or mammalian expression systems, to produce a purified, functional enzyme for research and drug development. Its recombinant form retains catalytic activity, enabling studies on enzyme kinetics, inhibitor screening, and structural analysis. Researchers utilize PTGS2 recombinant protein to investigate its role in diseases like colorectal cancer, neurodegenerative disorders, and cardiovascular conditions, where dysregulated prostaglandin signaling is implicated.
The protein’s structure, featuring a cyclooxygenase active site and a peroxidase domain, has been elucidated through X-ray crystallography, aiding in the design of selective inhibitors. Recombinant PTGS2 is also critical for validating drug specificity, minimizing off-target effects on PTGS1. which is essential for gastrointestinal and renal homeostasis.
In summary, PTGS2 recombinant protein serves as a vital tool for understanding inflammatory pathways, advancing targeted therapies, and exploring the enzyme’s dual roles in both physiological and pathological processes. Its application spans basic research, pharmaceutical development, and translational medicine.
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