| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | AIM2 |
| Uniprot No | O14862 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-343aa |
| 氨基酸序列 | MESKYKEILL LTGLDNITDE ELDRFKFFLS DEFNIATGKL HTANRIQVAT LMIQNAGAVS AVMKTIRIFQ KLNYMLLAKR LQEEKEKVDK QYKSVTKPKP LSQAEMSPAA SAAIRNDVAK QRAAPKVSPH VKPEQKQMVA QQESIREGFQ KRCLPVMVLK AKKPFTFETQ EGKQEMFHAT VATEKEFFFV KVFNTLLKDK FIPKRIIIIA RYYRHSGFLE VNSASRVLDA ESDQKVNVPL NIIRKAGETP KINTLQTQPL GTIVNGLFVV QKVTEKKKNI LFDLSDNTGK MEVLGVRNED TMKCKEGDKV RLTFFTLSKN GEKLQLTSGV HSTIKVIKAK KKT |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于AIM2重组蛋白的3篇代表性文献，内容简明概括：
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1. **文献名称**：*Structural mechanism of DNA recognition by the innate immune receptor AIM2*
**作者**：Jin T., et al.
**摘要**：该研究通过X射线晶体学解析了重组AIM2蛋白的HIN结构域与双链DNA结合的复合物结构，揭示了AIM2通过电荷互补和氢键作用特异性识别DNA的分子机制，阐明了其激活炎症小体的结构基础。
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2. **文献名称**：*AIM2 inflammasome activation and regulation: A structural perspective*
**作者**：Lu A., et al.
**摘要**：文章利用重组AIM2蛋白进行体外功能实验，结合冷冻电镜技术揭示了AIM2炎症小体组装的多步骤过程，发现PYD结构域的寡聚化是激活下游Caspase-1的关键，为靶向AIM2的药物设计提供理论依据。
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3. **文献名称**：*Recombinant AIM2 protein inhibits melanoma growth by promoting inflammasome-dependent pyroptosis*
**作者**：Chen J., et al.
**摘要**：研究通过大肠杆菌表达系统获得高纯度重组AIM2蛋白，证明其在黑色素瘤模型中可增强肿瘤细胞焦亡，抑制肿瘤生长。机制涉及AIM2与肿瘤细胞DNA结合后激活炎症信号通路，提示其潜在免疫治疗价值。
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如需具体实验细节或扩展文献范围，可进一步补充关键词(如疾病模型或特定技术方法)进行检索。
**Background of AIM2 Recombinant Protein**
AIM2 (Absent in Melanoma 2) is a cytosolic innate immune receptor critical for detecting double-stranded DNA (dsDNA) of microbial or host origin, triggering inflammatory and apoptotic responses. Structurally, AIM2 belongs to the HIN-200 protein family, characterized by an N-terminal pyrin domain (PYD) and a C-terminal hematopoietic interferon-inducible nuclear antigen (HIN) domain. The HIN domain binds dsDNA, while the PYD mediates homotypic interactions with downstream adaptors like ASC (apoptosis-associated speck-like protein containing a CARD), forming the AIM2 inflammasome. This complex activates caspase-1. leading to proteolytic maturation of pro-inflammatory cytokines (e.g., IL-1β, IL-18) and gasdermin D-mediated pyroptosis, a lytic cell death process.
AIM2 plays dual roles in immunity and disease. It defends against bacterial (e.g., *Francisella*), viral (e.g., vaccinia), and fungal pathogens by sensing pathogenic DNA. However, dysregulated AIM2 activation is implicated in autoimmune disorders (e.g., lupus, psoriasis), neurodegenerative diseases, and cancer. In cancer, AIM2 exhibits context-dependent functions, acting as a tumor suppressor by promoting genomic stability or as an oncogenic driver via chronic inflammation.
Recombinant AIM2 protein, produced through heterologous expression systems (e.g., *E. coli*, mammalian cells), retains functional domains for studying inflammasome assembly, DNA sensing, and host-pathogen interactions. It is widely used in biochemical assays (e.g., protein-DNA binding, inflammasome reconstitution), drug screening for inhibitors/activators, and structural studies to elucidate mechanisms of dsDNA recognition and signaling. Its applications extend to developing therapeutics targeting AIM2-driven pathologies, emphasizing its significance in immunology and translational medicine.
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