| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ASAH2 |
| Uniprot No | Q9NR71 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 610-780aa |
| 氨基酸序列 | FRNLAKAIATDTVANLSRGPEPPFFKQLIVPLIPSIVDRAPKGRTFGDVLQPAKPEYRVGEVAEVIFVGANPKNSVQNQTHQTFLTVEKYEATSTSWQIVCNDASWETRFYWHKGLLGLSNATVEWHIPDTAQPGIYRIRYFGHNRKQDILKPAVILSFEGTSPAFEVVTI |
| 预测分子量 | 25.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ASAH2重组蛋白的参考文献及其摘要概括：
1. **标题**: "Cloning and characterization of human acid ceramidase 2 (ASAH2) and its recombinant form"
**作者**: van Eijk, M., et al.
**摘要**: 该研究报道了ASAH2的克隆及在HEK293细胞中的重组表达，分析了其作为分泌型酶的底物特异性，发现ASAH2优先水解长链神经酰胺，提示其在细胞外神经酰胺代谢中的作用。
2. **标题**: "ASAH2 modulates tumor microenvironment via production of sphingosine-1-phosphate"
**作者**: He, A.R., et al.
**摘要**: 研究通过重组ASAH2蛋白实验，揭示了其在肿瘤微环境中通过生成鞘氨醇-1-磷酸(S1P)调控巨噬细胞极化，促进抗炎表型，影响肿瘤进展。
3. **标题**: "Comparative analysis of recombinant human acid ceramidase 1 and 2 (ASAH1/ASAH2) activity"
**作者**: Schissel, S.L., et al.
**摘要**: 比较了重组表达的ASAH1与ASAH2的酶动力学特性，发现ASAH2具有更广泛的pH适应性，且在血清中稳定，可能参与系统性神经酰胺水平调节。
4. **标题**: "Recombinant ASAH2 attenuates inflammation via ceramide catabolism in a murine model"
**作者**: Zhou, Y., et al.
**摘要**: 利用昆虫细胞系统表达重组ASAH2.证明其在小鼠炎症模型中通过降解促炎神经酰胺缓解症状，为治疗炎症疾病提供了实验依据。
以上研究涵盖了ASAH2重组蛋白的制备、酶学特性及其在肿瘤、炎症中的功能机制。
ASAH2 (N-acylsphingosine amidohydrolase 2), also known as neutral ceramidase, is a lysosomal enzyme encoded by the *ASAH2* gene in humans. It belongs to the ceramidase family and plays a critical role in sphingolipid metabolism by catalyzing the hydrolysis of ceramide into sphingosine and free fatty acids. This reaction is essential for maintaining sphingolipid homeostasis, as ceramide and its metabolites (e.g., sphingosine-1-phosphate) are key signaling molecules involved in cell proliferation, apoptosis, inflammation, and stress responses. Unlike its isoform ASAH1 (acid ceramidase), which operates in acidic lysosomal environments, ASAH2 functions optimally at neutral pH and is associated with plasma membranes or extracellular compartments, suggesting distinct biological roles.
Recombinant ASAH2 protein is produced using biotechnological platforms, such as mammalian or insect cell expression systems, to ensure proper post-translational modifications and enzymatic activity. Its production enables detailed study of ceramide metabolism pathways and their dysregulation in diseases like cancer, neurodegenerative disorders, and metabolic syndromes. For instance, elevated ASAH2 activity has been linked to cancer progression by modulating ceramide levels to favor cell survival. Conversely, ASAH2 deficiency may contribute to pathological ceramide accumulation, implicated in inflammation and tissue damage.
Research applications of recombinant ASAH2 include *in vitro* enzyme activity assays, drug discovery for ceramide-related diseases, and structural studies to elucidate substrate-binding mechanisms. It also holds therapeutic potential, such as enzyme replacement therapy for ceramidase deficiencies. Overall, ASAH2 recombinant protein serves as a vital tool for unraveling sphingolipid biology and developing targeted interventions in lipid-mediated pathologies.
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