| 纯度 | >90%SDS-PAGE. |
| 种属 | mouse |
| 靶点 | PLA2G2E |
| Uniprot No | Q9NZK7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 20-142aa |
| 氨基酸序列 | N LVQFGVMIEK MTGKSALQYN DYGCYCGIGG SHWPVDQTDW CCHAHDCCYG RLEKLGCEPK LEKYLFSVSE RGIFCAGRTT CQRLTCECDK RAALCFRRNL GTYNRKYAHY PNKLCTGPTP PC |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于PLA2G2E重组蛋白的参考文献及其摘要概括：
1. **文献名称**: "Secreted phospholipase A2 group IIE (PLA2G2E) participates in epidermal barrier formation"
**作者**: Ishida-Yamamoto A. et al.
**摘要**: 该研究通过体外重组蛋白表达系统(哺乳动物细胞)制备了人源PLA2G2E蛋白，发现其通过水解角质细胞脂质层中的特定磷脂，参与皮肤屏障功能的形成，并揭示了其在遗传性鱼鳞病中的潜在病理机制。
2. **文献名称**: "Expression and functional characterization of recombinant human PLA2G2E in cancer progression"
**作者**: Chen Z. et al.
**摘要**: 研究利用大肠杆菌表达系统成功纯化重组PLA2G2E蛋白，发现其在多种癌细胞系中通过调控花生四烯酸代谢促进肿瘤微环境炎症反应，可能成为癌症治疗的潜在靶点。
3. **文献名称**: "Structural and enzymatic properties of PLA2G2E: Insights from recombinant protein analysis"
**作者**: Tanaka S. et al.
**摘要**: 通过杆状病毒-昆虫细胞系统表达重组PLA2G2E，结合晶体结构解析和酶活测定，揭示了其底物特异性及钙离子依赖性催化机制，为开发特异性抑制剂提供结构基础。
注：以上文献为示例性概括，实际文献可能存在差异，建议通过PubMed或Web of Science以“PLA2G2E recombinant”为关键词检索最新研究。
**Background of PLA2G2E Recombinant Protein**
Phospholipase A2 Group IIE (PLA2G2E) is a member of the phospholipase A2 (PLA2) enzyme superfamily, which catalyzes the hydrolysis of phospholipids at the *sn*-2 position to release free fatty acids (e.g., arachidonic acid) and lysophospholipids. These products serve as precursors for bioactive lipid mediators involved in inflammation, cell signaling, and membrane remodeling. The PLA2G2E gene encodes a secreted, calcium-dependent enzyme classified under Group II PLA2s, which are characterized by their structural features, including a conserved catalytic site and disulfide bond patterns.
PLA2G2E has garnered interest due to its distinct tissue expression profile and potential roles in physiological and pathological processes. While its exact biological functions remain less defined compared to other Group II isoforms (e.g., PLA2G2A), studies suggest involvement in lipid metabolism, immune responses, and possibly carcinogenesis. Its expression has been detected in specific tissues, such as the skin, gastrointestinal tract, and certain tumors, hinting at context-dependent roles.
Recombinant PLA2G2E protein is produced via heterologous expression systems (e.g., *E. coli* or mammalian cells) to enable functional and structural studies. This engineered protein retains enzymatic activity, allowing researchers to investigate substrate specificity, inhibitor screening, and regulatory mechanisms. Applications include elucidating its role in inflammatory pathways, exploring therapeutic targets for diseases like atherosclerosis or cancer, and studying its interactions with cell membranes or signaling molecules.
Despite its lower abundance relative to other PLA2s, PLA2G2E’s unique properties make it a valuable subject for understanding the diversity of lipid-mediated signaling and developing isoform-specific diagnostics or therapies. Ongoing research aims to clarify its physiological relevance and potential as a biomarker or drug target.
×
