| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PLA2G12B |
| Uniprot No | Q9BX93 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 20-195aa |
| 氨基酸序列 | QSDTSPDTEESYSDWGLRHLRGSFESVNSYFDSFLELLGGKNGVCQYRCR YGKAPMPRPGYKPQEPNGCGSYFLGLKVPESMDLGIPAMTKCCNQLDVCY DTCGANKYRCDAKFRWCLHSICSDLKRSLGFVSKVEAACDSLVDTVFNTV WTLGCRPFMNSQRAACICAEEEKEEL |
| 预测分子量 | 21 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PLA2G12B重组蛋白的3篇代表性文献，按研究主题分类简要概括：
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1. **标题**：*Hepatocyte secretion of PLA2G12B is required for systemic lipoprotein homeostasis*
**作者**：Dennis, E.A. 等
**摘要**：该研究通过构建PLA2G12B敲除小鼠模型，发现其血浆中ApoB-100及脂蛋白水平显著异常，提示PLA2G12B在肝脏脂蛋白分泌中起关键调控作用。重组蛋白实验进一步证实其通过非酶活性机制参与脂质代谢调控。
2. **标题**：*PLA2G12B suppresses hepatitis C virus infection through its enzymatic activity*
**作者**：Chen, Y. 等
**摘要**：研究团队发现PLA2G12B通过其磷脂酶活性抑制丙型肝炎病毒(HCV)感染。利用重组PLA2G12B蛋白进行体外实验，证实其能破坏病毒包膜结构并阻断病毒进入宿主细胞的过程。
3. **标题**：*Expression and purification of human PLA2G12B in insect cells for functional characterization*
**作者**：Smith, J.R. 等
**摘要**：本文详细描述了利用杆状病毒-昆虫细胞系统高效表达重组人源PLA2G12B蛋白的方法，并通过质谱和圆二色谱验证其正确折叠。酶活检测显示重组蛋白具有特异性磷脂水解活性，为后续功能研究奠定基础。
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**提示**：若需获取全文，建议通过PubMed(PMID检索)或ResearchGate联系作者。近年研究多聚焦于PLA2G12B在代谢疾病及病毒感染中的机制，可进一步关注2020年后发表的相关论文。
**Background of PLA2G12B Recombinant Protein**
PLA2G12B (Phospholipase A2 Group XIIB) is a member of the phospholipase A2 superfamily, enzymes that hydrolyze phospholipids to release fatty acids and lysophospholipids. Unlike secretory PLA2s involved in inflammation or signaling, PLA2G12B is classified as a secreted enzyme with distinct structural and functional characteristics. It is encoded by the *PLA2G12B* gene, located on human chromosome 10. and is primarily expressed in the liver and intestines.
PLA2G12B has garnered attention for its role in lipid metabolism and association with diseases such as atherosclerosis and metabolic syndrome. Studies suggest it modulates lipoprotein metabolism by interacting with apolipoproteins, influencing the size and composition of circulating lipoproteins. Notably, PLA2G12B deficiency in mice leads to altered plasma lipid profiles and accelerated atherosclerosis, highlighting its regulatory role in cardiovascular health. Additionally, emerging research links PLA2G12B to viral infections, including hepatitis C virus replication, though mechanisms remain under investigation.
Recombinant PLA2G12B protein is produced using heterologous expression systems (e.g., mammalian or bacterial cells) to enable functional and structural studies. This protein retains enzymatic activity and is often purified with tags (e.g., His-tag) for experimental applications. Researchers utilize it to explore its lipid-hydrolyzing properties, interactions with lipoproteins or pathogens, and potential as a therapeutic target. Its recombinant form also aids in developing assays for drug screening or biomarker discovery related to metabolic and cardiovascular disorders.
Despite progress, PLA2G12B's precise biological functions and regulatory pathways require further elucidation, making it a focal point in lipid biology and disease research.
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