| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | Car12 |
| Uniprot No | Q8CI85 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-354aa |
| 氨基酸序列 | MPHRSLRATVVLLLVILKKQPSSSAPLNGSKWTYVGPAGEKNWSKKYPSCGGLLQSPIDLHSDILQYDASLAPLQFQGYNVSVEKLLNLTNDGHSVRLNLNSDMYIQGLQPHHYRAEQLHLHWGNRNDPHGSEHTVSGKHFAAELHIVHYNSDLYPDFSTASDKSEGLAVLAVLIEIGSANPSYDKIFSHLQHVKYKGQQVLIPGFNIEELLPESPGEYYRYEGSLTTPPCYPTVLWTVFRNPVQISQEQLLALETALYFTHMDDPTPREMINNFRQVQKFDERLVYISFRQGLLTDTGLSLGIILSVALAGVLGISIVLAVSIWLFKRKKSKKGDNKGVIYKPAIKKEAEVHA |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于Car12重组蛋白的模拟参考文献示例(仅供学术参考，具体文献需通过数据库验证)：
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1. **文献名称**: *"Expression and Purification of Recombinant Carbonic Anhydrase XII in E. coli for Functional Studies"*
**作者**: Smith A, et al.
**摘要**: 本研究报道了在大肠杆菌中高效表达和纯化重组Car12蛋白的方法，通过优化密码子和诱导条件获得可溶性蛋白，并验证了其碳酸酐酶活性，为后续酶学及抑制剂筛选提供基础。
2. **文献名称**: *"Role of CA XII in Tumor Acidosis: Insights from Recombinant Protein-Based Models"*
**作者**: Johnson R, et al.
**摘要**: 利用重组Car12蛋白模拟肿瘤微环境，发现其通过调节细胞外pH促进肿瘤细胞侵袭，提示Car12可能作为癌症治疗的潜在靶点。
3. **文献名称**: *"Structural Characterization of Recombinant Human CA XII Using X-ray Crystallography"*
**作者**: Lee S, et al.
**摘要**: 通过X射线晶体学解析了重组Car12的三维结构，揭示了其与已知抑制剂结合的活性位点特征，为设计特异性药物提供结构依据。
4. **文献名称**: *"Development of a High-Throughput Screening Assay for CA XII Inhibitors Using Recombinant Protein"*
**作者**: Müller C, et al.
**摘要**: 基于重组Car12蛋白建立高通量筛选平台，鉴定出多个新型抑制剂，并评估其在抗肿瘤中的应用潜力。
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**建议**：若需真实文献，可通过PubMed或Web of Science搜索关键词“CA XII recombinant”“Carbonic Anhydrase 12 expression”，并筛选近5年研究。部分关键作者包括S. Pastorekova(Car12发现者)或C.T. Supuran(CA抑制剂领域)。
**Background of CAR12 Recombinant Protein**
Carbonic anhydrase XII (CA12), encoded by the *CA12* gene, is a zinc-containing metalloenzyme belonging to the carbonic anhydrase family. It plays a critical role in regulating pH homeostasis by catalyzing the reversible hydration of carbon dioxide to bicarbonate and protons. Unlike other isoforms, CA12 is a transmembrane protein predominantly expressed in normal tissues such as the kidneys, colon, and reproductive organs. Its localization on the cell surface enables participation in extracellular pH regulation, a function linked to cellular processes like ion transport and metabolic adaptation.
CA12 has garnered attention in cancer research due to its overexpression in various tumors, including breast, renal, and cervical cancers. In hypoxic tumor microenvironments, CA12 helps maintain a neutral intracellular pH while acidifying the extracellular space, promoting tumor survival, invasion, and resistance to therapies. This pH modulation also influences immune evasion, making CA12 a potential therapeutic target.
Recombinant CA12 protein is produced using expression systems (e.g., *E. coli*, mammalian cells) to study its structure, function, and interactions. Purified recombinant CA12 retains enzymatic activity and is utilized in biochemical assays, inhibitor screening, and antibody development. Its applications extend to diagnostic research, as CA12 levels in bodily fluids or tissues may serve as biomarkers for certain cancers.
Current studies focus on designing CA12-specific inhibitors, such as monoclonal antibodies or small molecules, to disrupt its oncogenic role. However, challenges remain in ensuring selectivity over other CA isoforms to minimize off-target effects. Overall, recombinant CA12 protein serves as a vital tool for advancing both basic research and therapeutic strategies in pH-dependent pathologies.
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