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| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FGF8a |
| Uniprot No | P55075-2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 23-204aa |
| 氨基酸序列 | QHVREQSLVTDQLSRRLIRTYQLYSRTSGKHVQVLANKRINAMAEDGDPF AKLIVETDTFGSRVRVRGAETGLYICMNKKGKLIAKSNGKGKDCVFTEIV LENNYTALQNAKYEGWYMAFTRKGRPRKGSKTRQHQREVHFMKRLPRGHH TTEQSLRFEFLNYPPFTRSLRGSQRTWAPEPR |
| 预测分子量 | 21 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于FGF8a重组蛋白的文献信息及摘要概括：
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1. **文献名称**: *Fibroblast growth factor 8a regulates embryonic development and cell signaling*
**作者**: Sato T, et al.
**摘要**: 研究利用重组FGF8a蛋白在斑马鱼胚胎中验证其调控中脑和后脑发育的作用，证明其通过激活MAPK/ERK通路影响神经祖细胞增殖。
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2. **文献名称**: *Recombinant FGF8a induces branching morphogenesis in mouse mammary epithelial cells*
**作者**: Lu P, et al.
**摘要**: 该文献通过体外实验证明，重组FGF8a蛋白可激活FGFR1受体，促进小鼠乳腺上皮细胞的导管分支形成，提示其在器官形态发生中的关键作用。
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3. **文献名称**: *Functional characterization of FGF8a in craniofacial development using a recombinant protein approach*
**作者**: Trumpp A, et al.
**摘要**: 利用重组FGF8a蛋白处理鸡胚颅神经嵴细胞，发现其通过调控BMP和Wnt信号通路异常表达导致颅面骨骼发育缺陷，强调剂量依赖性效应。
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**备注**：以上文献信息为示例，实际引用时建议通过PubMed或Google Scholar以“FGF8a recombinant protein”为关键词检索最新文献，并核对具体实验模型(如斑马鱼、细胞系等)的适用性。
Fibroblast Growth Factor 8a (FGF8a) is a splice variant of the FGF8 gene, a member of the fibroblast growth factor (FGF) family known for its critical roles in embryonic development, tissue repair, and cellular signaling. FGF8a is a secreted protein that binds to FGF receptors (FGFRs) with heparan sulfate proteoglycans as co-factors, activating downstream pathways such as MAPK/ERK and PI3K/AKT to regulate cell proliferation, migration, and differentiation. It is particularly notable for its involvement in early embryogenesis, including neural tube patterning, limb bud formation, and midbrain-hindbrain boundary organization. Studies in model organisms, such as mice and zebrafish, highlight its importance in establishing body axes and organogenesis.
Recombinant FGF8a is produced using biotechnological methods, typically through expression in Escherichia coli or mammalian cell systems, followed by purification to ensure high bioactivity and low endotoxin levels. This engineered protein retains the functional properties of native FGF8a, making it a valuable tool for developmental biology research, stem cell differentiation protocols, and disease modeling. For example, it is used to study congenital disorders linked to FGF8 signaling defects, such as holoprosencephaly or limb malformations. Additionally, FGF8a's role in cancer progression, particularly in tumors with aberrant FGF signaling, has spurred interest in its therapeutic targeting. Researchers also utilize recombinant FGF8a to explore tissue regeneration strategies, leveraging its mitogenic effects in wound healing and neural repair contexts. Its controlled application in vitro and in vivo continues to advance our understanding of developmental mechanisms and potential clinical applications.
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