| 纯度 | >85% SDS-PAGE |
| 种属 | Human |
| 靶点 | BRICK1 |
| Uniprot No | Q8WUW1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-75aa |
| 氨基酸序列 | AGQEDPVQR EIHQDWANRE YIEIITSSIK KIADFLNSFD MSCRSRLATL NEKLTALERR IEYIEARVTK GETLT |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于BRICK1重组蛋白的假设性参考文献示例，基于其可能的研究方向及重组蛋白应用场景构建：
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1. **文献名称**：Structural Insights into BRICK1 Function within the WAVE Regulatory Complex
**作者**：Smith A, et al. (2005)
**摘要**：本研究通过在大肠杆菌中表达并纯化重组人源BRICK1蛋白，结合X射线晶体学分析其三维结构，揭示了BRICK1通过N端结构域与WAVE复合体其他组分(如SRA1)的关键相互作用，证实其在稳定复合体构象中的必要性，为解析细胞迁移机制提供结构基础。
2. **文献名称**：Recombinant BRICK1 Mediates ARP2/3 Activation in Cell Motility Assays
**作者**：Zhang L, et al. (2008)
**摘要**：作者利用昆虫杆状病毒系统表达并纯化功能性BRICK1重组蛋白，通过体外重建实验证明其与WAVE2、NAP1共同激活ARP2/3复合体，促进肌动蛋白聚合。进一步细胞实验表明，重组BRICK1的缺失导致细胞伪足形成受阻，强调其在迁移中的核心作用。
3. **文献名称**：Optimization of BRICK1 Recombinant Protein Expression in Mammalian Cells
**作者**：Johnson R, et al. (2012)
**摘要**：研究系统比较了哺乳动物HEK293细胞与大肠杆菌中BRICK1重组蛋白的表达效率，发现前者更适于保留蛋白翻译后修饰功能。通过优化载体设计及纯化流程，获得高纯度BRICK1.并验证其与Abi1的体外结合活性，为后续功能研究提供可靠工具。
4. **文献名称**：BRICK1 Knockdown Phenotypes Rescued by Exogenous Recombinant Protein Delivery
**作者**：Lee S, et al. (2010)
**摘要**：通过脂质体转递重组BRICK1蛋白至基因敲除细胞，成功恢复了因BRICK1缺失导致的WAVE复合体解聚及细胞铺展缺陷，证实外源重组蛋白的功能完整性，并揭示其通过C端结构域招募下游效应分子的分子机制。
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*注：上述文献为示例性内容，实际研究中请通过学术数据库检索真实发表论文。*
BRICK1 (also known as BRK1 or HSPC300) is a conserved subunit of the SCAR/WAVE (Suppressor of cAMP Receptor/WASP-family verprolin-homologous protein) regulatory complex, which plays a critical role in regulating actin cytoskeleton dynamics. This protein is essential for coordinating cell shape, motility, and membrane remodeling by activating the Arp2/3 complex to nucleate branched actin networks. The SCAR/WAVE complex, including BRICK1. acts downstream of Rac GTPase signaling, linking extracellular cues to intracellular actin reorganization.
First identified in *Dictyostelium* and later characterized in plants, mammals, and other eukaryotes, BRICK1 is indispensable for embryogenesis, tissue development, and cellular processes such as cytokinesis and endocytosis. In plants, BRICK1 homologs regulate cell morphogenesis, including root hair and pavement cell patterning. Mutations in BRICK1 are associated with developmental defects, highlighting its evolutionary importance.
Recombinant BRICK1 proteins are produced using expression systems like *E. coli* or mammalian cells to study its structure-function relationships, interaction partners, and regulatory mechanisms. Structural studies reveal that BRICK1 contains an N-terminal helical domain and a C-terminal region critical for binding to other SCAR/WAVE subunits (e.g., Sra1. Nap1. Abi2). Its small size (~10 kDa) and conserved sequence make it a focus for biochemical and biophysical analyses.
Research on recombinant BRICK1 has advanced understanding of cytoskeletal disorders, cancer metastasis (due to dysregulated cell migration), and neurodevelopmental conditions. It also serves as a tool for screening therapeutic agents targeting actin-dependent processes. Despite its minimal size, BRICK1’s role in stabilizing the SCAR/WAVE complex underscores its significance in cellular architecture and disease pathways.
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