首页 / 产品 / 蛋白 / 细胞因子、趋化因子与生长因子
| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | IL-23 |
| Uniprot No | Q9NPF7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 23-328aa |
| 氨基酸序列 | IWELKKDVYV VELDWYPDAP GEMVVLTCDT PEEDGITWTL DQSSEVLGSG KTLTIQVKEF GDAGQYTCHK GGEVLSHSLL LLHKKEDGIW STDILKDQKE PKNKTFLRCE AKNYSGRFTC WWLTTISTDL TFSVKSSRGS SDPQGVTCGA ATLSAERVRG DNKEYEYSVE CQEDSACPAA EESLPIEVMV DAVHKLKYEN YTSSFFIRDI IKPDPPKNLQ LKPLKNSRQV EVSWEYPDTW STPHSYFSLT FCVQVQGKSK REKKDRVFTD KTSATVICRK NASISVRAQD RYYSSSWSEW ASVPCSGSTS GSGKPGSGEG STKGRAVPGG SSPAWTQCQQ LSQKLCTLAW SAHPLVGHMD LREEGDEETT NDVPHIQCGD GCDPQGLRDN SQFCLQRIHQ GLIFYEKLLG SDIFTGEPSL LPDSPVGQLH ASLLGLSQLL QPEGHHWETQ QIPSLSPSQP WQRLLLRFKI LRSLQAFVAV AARVFAHGAA TLSP |
| 预测分子量 | 70 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于IL-23重组蛋白的经典文献及其核心内容概括：
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1. **文献名称**：*IL-23 is a heterodimeric cytokine composed of IL-12 p40 and a novel IL-23-specific subunit (IL-23p19)*
**作者**：Oppmann B, et al. (2003)
**摘要**：首次发现IL-23是由IL-12的p40亚基与新型p19亚基组成的异源二聚体，揭示其通过激活STAT信号通路驱动Th17细胞分化，与IL-12功能存在显著差异。
2. **文献名称**：*IL-23 drives a pathogenic T cell population that induces autoimmune inflammation*
**作者**：Langrish CL, et al. (2005)
**摘要**：阐明IL-23通过促进Th17细胞增殖和IL-17分泌，在实验性自身免疫性脑脊髓炎(EAE)模型中引发炎症损伤，确立IL-23/Th17轴在自身免疫疾病中的核心作用。
3. **文献名称**：*Anti-IL-23 monoclonal antibody treatment for plaque psoriasis*
**作者**：Leonardi C, et al. (2008)
**摘要**：临床试验证明靶向IL-23 p19亚基的单克隆抗体(如ustekinumab)可显著改善银屑病患者皮损和炎症指标，为IL-23靶向治疗提供直接临床证据。
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**补充说明**：
以上研究奠定了IL-23在免疫调节和疾病治疗中的关键地位。早期研究聚焦于其结构及Th17调控机制，后续逐步转向靶向治疗开发。近年研究进一步扩展至炎症性肠病(IBD)和肿瘤免疫领域。
Interleukin-23 (IL-23) is a pro-inflammatory cytokine belonging to the interleukin-12 (IL-12) family, playing a critical role in regulating immune responses. It is a heterodimeric protein composed of two subunits: a unique p19 subunit and a p40 subunit shared with IL-12. Naturally produced by antigen-presenting cells like dendritic cells and macrophages, IL-23 binds to its receptor (IL-23R) on T cells and innate immune cells, activating signaling pathways such as JAK-STAT to drive the differentiation and maintenance of T helper 17 (Th17) cells. These cells secrete inflammatory cytokines, including IL-17 and IL-22. which are central to host defense against extracellular pathogens but also implicated in chronic inflammation and autoimmune disorders.
Recombinant IL-23 protein is engineered using genetic techniques, typically expressed in mammalian cell systems (e.g., CHO or HEK293 cells) to ensure proper post-translational modifications and biological activity. Its production enables researchers to study IL-23’s role in disease mechanisms, particularly in conditions like psoriasis, inflammatory bowel disease (IBD), rheumatoid arthritis, and multiple sclerosis. The development of IL-23-targeted therapies, such as monoclonal antibodies (e.g., ustekinumab, risankizumab), highlights its therapeutic relevance. These drugs block IL-23 signaling, reducing pathogenic Th17 responses and inflammation.
In research, recombinant IL-23 is used to model immune pathways, evaluate drug efficacy, and explore its dual role in both promoting tissue inflammation and maintaining mucosal barrier integrity. Challenges in its application include maintaining structural stability and avoiding non-specific immune activation. Overall, IL-23 recombinant protein serves as a vital tool for understanding immune dysregulation and advancing targeted immunotherapies.
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