| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ACP1 |
| Uniprot No | P24666 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-158aa |
| 氨基酸序列 | MAEQATKSVLFVCLGNICRSPIAEAVFRKLVTDQNISENWRVDSAATSGYEIGNPPDYRGQSCMKRHGIPMSHVARQITKEDFATFDYILCMDESNLRDLNRKSNQVKTCKAKIELLGSYDPQKQLIIEDPYYGNDSDFETVYQQCVRCCRAFLEKAH |
| 预测分子量 | 45.0kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ACP1重组蛋白的3篇模拟参考文献示例(文献为虚构，仅供参考)：
1. **文献名称**：*"Recombinant Human ACP1: Expression in E. coli and Enzymatic Characterization"*
**作者**：Zhang Y, et al.
**摘要**：该研究报道了人源ACP1基因在大肠杆菌中的重组表达与纯化，通过His标签亲和层析获得高纯度蛋白，并验证其酸性磷酸酶活性，证实重组蛋白在体外对磷酸酪氨酸底物的水解功能。
2. **文献名称**：*"Structural Insights into ACP1 Phosphatase by X-ray Crystallography"*
**作者**：Lee S, et al.
**摘要**：本文解析了重组ACP1蛋白的晶体结构，揭示了其催化活性中心的关键氨基酸残基，并探讨了ACP1在低pH环境下的底物特异性机制，为靶向ACP1的药物设计提供结构基础。
3. **文献名称**：*"Role of Recombinant ACP1 in T-Cell Signaling and Autoimmune Disease"*
**作者**：Müller R, et al.
**摘要**：研究利用哺乳动物细胞表达的重组ACP1蛋白，发现其通过去磷酸化STAT3调控T细胞活化，提示ACP1可能成为自身免疫性疾病治疗的潜在靶点。
注：以上文献为模拟示例，实际文献需通过PubMed、Web of Science等学术平台检索关键词“ACP1 recombinant protein”或“ACP1 phosphatase recombinant”获取。
ACP1 (acid phosphatase 1), also known as low molecular weight protein tyrosine phosphatase (LMW-PTP), is a ubiquitously expressed enzyme that plays critical roles in cellular signaling and metabolic regulation. It belongs to the protein tyrosine phosphatase (PTP) family, which modulates phosphorylation-dependent signaling pathways by dephosphorylating tyrosine residues in target proteins. ACP1 specifically regulates key cellular processes, including cell proliferation, differentiation, and apoptosis, by interacting with signaling molecules such as receptors for insulin, platelet-derived growth factor (PDGF), and other tyrosine kinase-associated pathways. Dysregulation of ACP1 has been linked to metabolic disorders, cancer, and immune-related diseases, highlighting its therapeutic and diagnostic potential.
Recombinant ACP1 protein is produced using biotechnological methods, typically through heterologous expression in bacterial (e.g., *E. coli*) or mammalian cell systems. The recombinant form retains the enzymatic activity of the native protein, enabling researchers to study its structure, catalytic mechanisms, and interactions with substrates or inhibitors. Production involves cloning the ACP1 gene into an expression vector, followed by purification via affinity chromatography (e.g., His-tag systems) and quality validation using techniques like SDS-PAGE, Western blotting, and enzymatic assays.
Studies leveraging recombinant ACP1 have advanced our understanding of its role in insulin signaling, tumor suppression, and oxidative stress responses. It serves as a tool for drug discovery, particularly in designing inhibitors for cancer therapy or activators for metabolic disorders. Additionally, recombinant ACP1 is used in diagnostic applications to detect phosphatase activity in biological samples. Ongoing research focuses on elucidating its isoform-specific functions and exploring its potential as a biomarker or therapeutic target in diseases like diabetes, leukemia, and solid tumors.
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