| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | UBA1 |
| Uniprot No | P22314 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1058aa |
| 氨基酸序列 | MSSSPLSKKRRVSGPDPKPGSNCSPAQSVLSEVPSVPTNGMAKNGSEADIDEGLYSRQLYVLGHEAMKRLQTSSVLVSGLRGLGVEIAKNIILGGVKAVTLHDQGTAQWADLSSQFYLREEDIGKNRAEVSQPRLAELNSYVPVTAYTGPLVEDFLSGFQVVVLTNTPLEDQLRVGEFCHNRGIKLVVADTRGLFGQLFCDFGEEMILTDSNGEQPLSAMVSMVTKDNPGVVTCLDEARHGFESGDFVSFSEVQGMVELNGNQPMEIKVLGPYTFSICDTSNFSDYIRGGIVSQVKVPKKISFKSLVASLAEPDFVVTDFAKFSRPAQLHIGFQALHQFCAQHGRPPRPRNEEDAAELVALAQAVNARALPAVQQNNLDEDLIRKLAYVAAGDLAPINAFIGGLAAQEVMKACSGKFMPIMQWLYFDALECLPEDKEVLTEDKCLQRQNRYDGQVAVFGSDLQEKLGKQKYFLVGAGAIGCELLKNFAMIGLGCGEGGEIIVTDMDTIEKSNLNRQFLFRPWDVTKLKSDTAAAAVRQMNPHIRVTSHQNRVGPDTERIYDDDFFQNLDGVANALDNVDARMYMDRRCVYYRKPLLESGTLGTKGNVQVVIPFLTESYSSSQDPPEKSIPICTLKNFPNAIEHTLQWARDEFEGLFKQPAENVNQYLTDPKFVERTLRLAGTQPLEVLEAVQRSLVLQRPQTWADCVTWACHHWHTQYSNNIRQLLHNFPPDQLTSSGAPFWSGPKRCPHPLTFDVNNPLHLDYVMAAANLFAQTYGLTGSQDRAAVATFLQSVQVPEFTPKSGVKIHVSDQELQSANASVDDSRLEELKATLPSPDKLPGFKMYPIDFEKDDDSNFHMDFIVAASNLRAENYDIPSADRHKSKLIAGKIIPAIATTTAAVVGLVCLELYKVVQGHRQLDSYKNGFLNLALPFFGFSEPLAAPRHQYYNQEWTLWDRFEVQGLQPNGEEMTLKQFLDYFKTEHKLEITMLSQGVSMLYSFFMPAAKLKERLDQPMTEIVSRVSKRKLGRHVRALVLELCCNDESGEDVEVPYVRYTIR |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于UBA1重组蛋白的3篇参考文献的简要总结(文献标题与作者为虚构示例，供参考)：
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1. **标题**：*Recombinant Expression and Functional Characterization of Human UBA1 in E. coli*
**作者**：Smith J, et al.
**摘要**：研究通过大肠杆菌系统成功表达并纯化重组人源UBA1蛋白，优化了表达条件以提高溶解度，并证实其泛素激活酶活性，为体外泛素化研究提供工具。
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2. **标题**：*Structural Insights into UBA1 Activation and Inhibition by Small Molecules*
**作者**：Lee C, et al.
**摘要**：利用重组UBA1蛋白进行X射线晶体学分析，解析了其与ATP和抑制剂TAK-243结合的复合物结构，揭示了酶活调控机制及药物开发潜力。
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3. **标题**：*UBA1 Mutations Alter Ubiquitination Dynamics in VEXAS Syndrome*
**作者**：Garcia R, et al.
**摘要**：通过重组突变型UBA1蛋白(如p.M41T)的功能研究，发现其导致泛素化异常及蛋白酶体功能失调，阐明了VEXAS综合征的分子病理机制。
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如需真实文献，建议在PubMed或Google Scholar以“UBA1 recombinant expression”、“UBA1 structural analysis”等关键词检索。
UBA1 is a critical enzyme in the ubiquitin-proteasome system (UPS), responsible for initiating ubiquitination—a post-translational modification that regulates protein degradation, cellular signaling, and DNA repair. As the primary E1 ubiquitin-activating enzyme, UBA1 catalyzes the ATP-dependent activation of ubiquitin and its transfer to E2 conjugating enzymes, a rate-limiting step in the ubiquitination cascade. Its structure includes adenylation, thioester bond-forming, and C-terminal ubiquitin-fold domains, which coordinate ATP binding, ubiquitin activation, and interactions with E2 enzymes.
Dysregulation of UBA1 is linked to severe human diseases. Somatic mutations in UBA1. particularly in hematopoietic stem cells, are associated with VEXAS syndrome (Vacuoles, E1 enzyme, X-linked, Autoinflammatory, Somatic), a recently identified adult-onset inflammatory disorder with hematologic manifestations. Additionally, UBA1 mutations are implicated in spinal muscular atrophy (SMA) and certain cancers, highlighting its role in maintaining cellular homeostasis.
Recombinant UBA1 proteins are engineered using expression systems (e.g., mammalian, bacterial, or insect cells) to study its biochemical functions, structural dynamics, and disease-related mutations. These proteins are purified via affinity tags (e.g., His-tag) and used in enzymatic assays, drug screening, and structural studies (e.g., X-ray crystallography). Researchers also utilize recombinant UBA1 to investigate mechanisms underlying UPS dysfunction and to develop targeted therapies, such as small-molecule inhibitors for cancer or activators for neurodegenerative diseases. Its recombinant form is indispensable for deciphering ubiquitination pathways and advancing precision medicine approaches.
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