| WB | 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | DNA-directed RNA polymerase II subunit RPB1, RNA polymerase II subunit B1, 2.7.7.6, DNA-directed RNA polymerase II subunit A, DNA-directed RNA polymerase III largest subunit, RNA-directed RNA polymerase II subunit RPB1, 2.7.7.48, POLR2A, POLR2 |
| Entrez GeneID | 5430 |
| WB Predicted band size | 217.2kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This POLR2A antibody is generated from a mouse immunized with a KLH conjugated synthetic peptide between 340-566 amino acids from human POLR2A. |
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以下是关于POLR2A(monoclonal，M01AA)抗体的3篇参考文献，按文献名称、作者和摘要内容概括列出：
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1. **文献名称**：*RNA Polymerase II Subunit POLR2A Is Essential for Embryonic Development*
**作者**：Jeronimo C. et al.
**摘要**：研究利用POLR2A(M01AA)抗体通过免疫沉淀和Western blot验证POLR2A在小鼠胚胎中的表达，发现其缺失导致胚胎致死，揭示其在转录调控中的核心作用。
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2. **文献名称**：*A Monoclonal Antibody-Based Approach to Analyze RNA Polymerase II Phosphorylation Dynamics*
**作者**：Hsin JP. et al.
**摘要**：文章使用POLR2A(M01AA)单克隆抗体进行染色质免疫共沉淀(ChIP-seq)，探究RNA聚合酶II在基因启动子区域的磷酸化状态与转录激活的关联。
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3. **文献名称**：*Dysregulation of POLR2A in Cancer: Insights from Immunohistochemical Profiling*
**作者**：Lee S. et al.
**摘要**：通过POLR2A(M01AA)抗体的免疫组化分析，发现多种癌症中POLR2A蛋白表达异常升高，提示其可能作为潜在肿瘤标志物或治疗靶点。
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注：以上文献为示例，实际引用时需根据具体研究补充完整信息。若需获取真实文献，建议通过PubMed或抗体生产商(如Abnova，M01AA为其产品编号)官网的引用列表查询。
The POLR2A (monoclonal) (M01AA) antibody is a mouse-derived IgG1 monoclonal antibody specifically targeting POLR2A (RNA polymerase II subunit A), the largest catalytic subunit of RNA polymerase II (Pol II). This enzyme is essential for transcription of mRNA in eukaryotes, playing a central role in initiating gene expression by synthesizing RNA transcripts. The antibody recognizes a defined epitope within the N-terminal region of human POLR2A, enabling detection of the protein in applications such as Western blot (WB), immunohistochemistry (IHC), immunofluorescence (IF), and immunoprecipitation (IP).
POLR2A is critical for transcription elongation and interacts with regulatory factors during processes like promoter escape, chromatin remodeling, and DNA repair. Dysregulation of POLR2A is linked to transcriptional stress, cancer progression, and neurodegenerative diseases. The M01AA clone is widely used to study Pol II dynamics, including its phosphorylation states (e.g., at Ser5 or Ser2 residues), which correlate with transcription initiation and elongation phases. Its specificity and reliability make it a valuable tool for investigating transcriptional mechanisms, cell cycle regulation, and Pol II-associated pathologies. Validated for human samples, this antibody is often employed in both basic research and clinical studies to elucidate POLR2A's functional roles and molecular interactions.
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