Rabbit Polyclonal RPS2 Antibody

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     All lanes : Anti-RPS2 Antibody (C-Term) at 1:2000 dilution Lane 1: Jurkat whole cell lysate Lane 2: A549 whole cell lysate Lane 3: HepG2 whole cell lysate Lane 4: NIH/3T3 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG,  (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 31 kDa Blocking/Dilution buffer: 5% NFDM/TBST.    

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     Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervical epithelial adenocarcinoma cell line) cells labeling RPS2 with P34368 at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-rabbit IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm and nucleus staining on HeLa cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin at 1/100 dilution (red). The nuclear counter stain is DAPI (blue).    

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     Overlay histogram showing Hela cells stained with P34368(green line).  The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min.  The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (P34368,  1:25 dilution) for 60 min at 37ºC.  The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37ºC.  Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions.  Acquisition of >10, 000 events was performed.    

The RPS2 antibody is designed to detect ribosomal protein S2 (RPS2), a component of the 40S ribosomal subunit involved in mRNA translation and protein synthesis. As part of the ribosome, RPS2 plays a critical role in cellular homeostasis, growth, and stress response. Dysregulation of RPS2 has been implicated in various pathologies, including cancers, autoimmune disorders, and viral infections. For example, RPS2 overexpression is observed in certain malignancies, where it may promote tumor progression by enhancing ribosome biogenesis. In autoimmune contexts, anti-RPS2 autoantibodies have been identified in systemic lupus erythematosus (SLE) patients, suggesting its potential role as an autoantigen.

Studies also highlight RPS2's interaction with viral RNA, such as in hepatitis C and HIV, where it may facilitate viral replication. Researchers utilize RPS2 antibodies in techniques like Western blotting, immunohistochemistry, and immunofluorescence to investigate its expression patterns, subcellular localization, and molecular interactions. These tools help unravel its contributions to disease mechanisms and therapeutic targeting. However, conflicting findings regarding its precise pathological roles underscore the need for further research. The RPS2 antibody thus serves as a vital reagent for exploring ribosome-related biology and its implications in human health and disease.