| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PDGFRB |
| Uniprot No | P09619 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 901-1106aa |
| 氨基酸序列 | GTPYPELPMNEQFYNAIKRGYRMAQPAHASDEIYEIMQKCWEEKFEIRPPFSQLVLLLERLLGEGYKKKYQQVDEEFLRSDHPAILRSQARLPGFHGLRSPLDTSSVLYTAVQPNEGDNDYIIPLPDPKPEVADEGPLEGSPSLASSTLNEVNTSSTISCDSPLEPQDEPEPEPQLELQVEPEPELEQLPDSGCPAPRAEAEDSFL |
| 预测分子量 | 54.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PDGFRB重组蛋白的3篇示例参考文献(注：文献信息为模拟示例，实际引用请通过学术数据库核实)：
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1. **文献名称**：Structural insights into PDGFRβ activation by ligand binding
**作者**：Shimizu A, et al.
**摘要**：该研究通过重组表达PDGFRβ的胞外域，利用X射线晶体学解析了其与PDGF-BB结合的复合物结构，揭示了受体二聚化及配体特异性结合的分子机制，为靶向PDGFRβ的药物设计提供了结构基础。
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2. **文献名称**：Recombinant PDGFRβ kinase domain production and its inhibitory screening
**作者**：Oefner C, et al.
**摘要**：作者通过原核系统高效表达并纯化了PDGFRβ的酪氨酸激酶结构域，建立了体外激酶活性检测体系，筛选出小分子抑制剂，验证了其在抗肿瘤细胞增殖中的作用。
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3. **文献名称**：Functional analysis of PDGFRβ mutations in cardiovascular diseases
**作者**：Heldin CH, et al.
**摘要**：利用重组PDGFRβ蛋白，研究了家族性特发性基底节钙化症相关突变对受体信号传导的影响，发现部分突变导致配体非依赖性激活，提示其在疾病发生中的潜在机制。
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**建议**：实际文献可通过PubMed、Web of Science等平台以关键词“PDGFRB recombinant protein”或“PDGFRB expression”检索，重点关注蛋白功能、结构解析及疾病应用方向的研究。
**Background of PDGFRB Recombinant Protein**
Platelet-Derived Growth Factor Receptor Beta (PDGFRB) is a transmembrane receptor tyrosine kinase that plays a critical role in regulating cell proliferation, migration, survival, and angiogenesis. It binds to platelet-derived growth factors (PDGFs), particularly PDGF-BB, initiating downstream signaling cascades such as MAPK, PI3K/Akt, and JAK/STAT pathways. PDGFRB is essential in embryonic development, tissue repair, and maintaining vascular integrity. Dysregulation of PDGFRB signaling, including mutations or overexpression, is linked to cancers (e.g., gliomas, gastrointestinal stromal tumors), fibrotic disorders, and rare genetic conditions like PDGFRB-related infantile myofibromatosis or idiopathic basal ganglia calcification.
Recombinant PDGFRB protein is engineered in vitro using expression systems (e.g., mammalian, insect cells) to produce soluble, bioactive forms of the receptor’s extracellular or intracellular domains. This protein retains ligand-binding or kinase activity, enabling functional studies. Researchers use it to investigate PDGF/PDGFRB interactions, screen inhibitors for therapeutic development, or model pathogenic mutations. High-purity recombinant PDGFRB, often purified via affinity chromatography, serves as a critical tool in structural studies (e.g., crystallography) and diagnostic assays.
Its applications span drug discovery, cancer research, and understanding molecular mechanisms of PDGFRB-associated diseases, highlighting its value in both basic science and translational medicine.
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