| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SELP |
| Uniprot No | P16109 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 42-771aa |
| 氨基酸序列 | WTYHYSTKAY SWNISRKYCQ NRYTDLVAIQ NKNEIDYLNK VLPYYSSYYW IGIRKNNKTW TWVGTKKALT NEAENWADNE PNNKRNNEDC VEIYIKSPSA PGKWNDEHCL KKKHALCYTA SCQDMSCSKQ GECLETIGNY TCSCYPGFYG PECEYVRECG ELELPQHVLM NCSHPLGNFS FNSQCSFHCT DGYQVNGPSK LECLASGIWT NKPPQCLAAQ CPPLKIPERG NMTCLHSAKA FQHQSSCSFS CEEGFALVGP EVVQCTASGV WTAPAPVCKA VQCQHLEAPS EGTMDCVHPL TAFAYGSSCK FECQPGYRVR GLDMLRCIDS GHWSAPLPTC EAISCEPLES PVHGSMDCSP SLRAFQYDTN CSFRCAEGFM LRGADIVRCD NLGQWTAPAP VCQALQCQDL PVPNEARVNC SHPFGAFRYQ SVCSFTCNEG LLLVGASVLQ CLATGNWNSV PPECQAIPCT PLLSPQNGTM TCVQPLGSSS YKSTCQFICD EGYSLSGPER LDCTRSGRWT DSPPMCEAIK CPELFAPEQG SLDCSDTRGE FNVGSTCHFS CDNGFKLEGP NNVECTTSGR WSATPPTCKG IASLPTPGLQ CPALTTPGQG TMYCRHHPGT FGFNTTCYFG CNAGFTLIGD STLSCRPSGQ WTAVTPACRA VKCSELHVNK PIAMNCSNLW GNFSYGSICS FHCLEGQLLN GSAQTACQEN GHWSTTVPTC QAGPLTIQEA |
| 预测分子量 | 80 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SELP重组蛋白的3-4篇参考文献示例(内容为虚构，仅供格式参考)：
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1. **文献名称**：*Recombinant Expression and Functional Analysis of Human P-Selectin Glycoprotein Ligand-1 in Inflammatory Models*
**作者**：Smith A, Brown C, Lee T.
**摘要**：研究通过哺乳动物表达系统成功制备重组人SELP蛋白，验证其与P-selectin的特异性结合能力，并证明其在体外炎症模型中抑制白细胞滚动黏附的作用。
2. **文献名称**：*Engineering a SELP-IgG Fusion Protein for Targeted Inhibition of Thrombosis*
**作者**：Zhang L, Wang H, Patel K.
**摘要**：构建SELP与免疫球蛋白Fc段的融合蛋白，通过结构优化增强其稳定性，并在血栓形成小鼠模型中展示其通过阻断血小板-P-selectin相互作用减轻血管阻塞的效果。
3. **文献名称**：*SELP-Based Nanoparticles for Drug Delivery to Inflamed Endothelium*
**作者**：Johnson R, Gupta S, Martinez E.
**摘要**：利用重组SELP蛋白的靶向性设计纳米颗粒，负载抗炎药物后在小鼠动脉粥样硬化模型中实现病灶特异性聚集，显著降低斑块炎症反应。
4. **文献名称**：*Optimized Production of Soluble SELP in Prokaryotic Systems and Its Therapeutic Application in Sepsis*
**作者**：Wang Y, Chen X, Liu F.
**摘要**：优化大肠杆菌表达体系实现SELP蛋白的可溶性高效表达，纯化产物在败血症模型中通过调控中性粒细胞浸润改善多器官功能障碍。
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注：以上文献信息为模拟生成，实际引用时请以真实出版物为准。
**Background of SELP Recombinant Protein**
SELP (P-selectin) is a cell adhesion molecule belonging to the selectin family, which plays a critical role in mediating leukocyte rolling and recruitment during inflammatory responses and thrombotic events. Expressed on activated endothelial cells and platelets, SELP interacts with ligands like PSGL-1 on leukocytes, facilitating transient adhesion under shear stress. This interaction is vital for immune surveillance and pathological conditions such as atherosclerosis, sepsis, and thrombosis.
Recombinant SELP protein is engineered using biotechnological platforms (e.g., mammalian or bacterial expression systems) to produce purified, functional forms of the protein for research and therapeutic applications. Structurally, SELP consists of an N-terminal lectin domain, an epidermal growth factor (EGF)-like module, multiple complement regulatory repeats, a transmembrane region, and a cytoplasmic tail. Recombinant versions often exclude transmembrane domains to generate soluble proteins for in vitro studies.
In research, SELP recombinant proteins are used to study leukocyte-endothelium interactions, screen inhibitory compounds (e.g., anti-inflammatory drugs), or develop diagnostic tools for vascular diseases. Its therapeutic potential includes targeting SELP-ligand interactions to mitigate inflammation or thrombosis. Modified variants (e.g., Fc-fusion proteins) may enhance stability or prolong half-life in vivo.
Overall, SELP recombinant proteins serve as essential tools for unraveling vascular biology mechanisms and advancing translational strategies in inflammatory and thrombotic disorders.
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