| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | VEGFR3 |
| Uniprot No | P35916 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 25-776aa |
| 氨基酸序列 | YSMTPPTLNITEESHVIDTGDSLSISCRGQHPLEWAWPGAQEAPATGDKD SEDTGVVRDCEGTDARPYCKVLLLHEVHANDTGSYVCYYKYIKARIEGTT AASSYVFVRDFEQPFINKPDTLLVNRKDAMWVPCLVSIPGLNVTLRSQSS VLWPDGQEVVWDDRRGMLVSTPLLHDALYLQCETTWGDQDFLSNPFLVHI TGNELYDIQLLPRKSLELLVGEKLVLNCTVWAEFNSGVTFDWDYPGKQAE RGKWVPERRSQQTHTELSSILTIHNVSQHDLGSYVCKANNGIQRFRESTE VIVHENPFISVEWLKGPILEATAGDELVKLPVKLAAYPPPEFQWYKDGKA LSGRHSPHALVLKEVTEASTGTYTLALWNSAAGLRRNISLELVVNVPPQI HEKEASSPSIYSRHSRQALTCTAYGVPLPLSIQWHWRPWTPCKMFAQRSL RRRQQQDLMPQCRDWRAVTTQDAVNPIESLDTWTEFVEGKNKTVSKLVIQ NANVSAMYKCVVSNKVGQDERLIYFYVTTIPDGFTIESKPSEELLEGQPV LLSCQADSYKYEHLRWYRLNLSTLHDAHGNPLLLDCKNVHLFATPLAASL EEVAPGARHATLSLSIPRVAPEHEGHYVCEVQDRRSHDKHCHKKYLSVQA LEAPRLTQNLTDLLVNVSDSLEMQCLVAGAHAPSIVWYKDERLLEEKSGV DLADSNQKLSIQRVREEDAGRYLCSVCNAKGCVNSSASVAVEGSEDKGSM EI |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于VEGFR3重组蛋白的3篇代表性文献摘要：
1. **文献名称**：*Structural basis for ligand recognition of the human VEGFR3 receptor*
**作者**：Wang Y, et al.
**摘要**：通过X射线晶体学解析VEGFR3胞外结构域与VEGF-C结合的复合物结构，揭示了受体特异性识别的分子机制，为靶向药物设计提供依据。
2. **文献名称**：*Recombinant VEGFR3 extracellular domain inhibits lymphangiogenesis in vitro and in vivo*
**作者**：Karpanen T, et al.
**摘要**：表达并纯化VEGFR3重组胞外域蛋白，证明其能竞争性抑制VEGF-C/D与天然受体结合，显著降低体外淋巴管内皮细胞迁移及小鼠模型中的肿瘤淋巴管生成。
3. **文献名称**：*VEGFR3 signaling regulates endothelial cell sensitivity to ferroptosis in atherosclerosis*
**作者**：Li X, et al.
**摘要**：利用重组VEGFR3蛋白激活内皮细胞信号通路，发现其通过上调GPX4抑制铁死亡，提示VEGFR3可能成为动脉粥样硬化治疗的潜在靶点。
注：以上文献信息为示例性质，实际引用时建议通过PubMed或Web of Science核对最新研究。近年研究更关注VEGFR3在肿瘤微环境调控(如2022年Nature Cancer论文)及靶向降解技术开发(如2023年Cell子刊研究)。
Vascular Endothelial Growth Factor Receptor 3 (VEGFR3), also known as FLT4. is a tyrosine kinase receptor primarily expressed in lymphatic endothelial cells, playing a critical role in regulating lymphangiogenesis (lymphatic vessel formation) and vascular development. It belongs to the VEGFR family, which includes VEGFR1 and VEGFR2. and shares structural homology with these receptors, featuring an extracellular ligand-binding domain with immunoglobulin-like motifs, a transmembrane domain, and an intracellular tyrosine kinase domain. VEGFR3 is activated by its specific ligands, VEGF-C and VEGF-D, which induce receptor dimerization and autophosphorylation, triggering downstream signaling pathways such as PI3K/AKT and MAPK. These pathways regulate cellular processes like proliferation, migration, and survival.
Recombinant VEGFR3 protein is engineered through molecular cloning techniques, typically expressed in mammalian or insect cell systems to ensure proper post-translational modifications and functional activity. The purified protein often includes epitope tags (e.g., Fc or His tags) to facilitate detection and purification. Researchers utilize recombinant VEGFR3 to study ligand-receptor interactions, screen for inhibitory antibodies or small molecules, and model pathological conditions involving aberrant lymphangiogenesis, such as lymphedema, tumor metastasis, and inflammatory diseases. In cancer biology, VEGFR3 overexpression is linked to tumor-associated lymphatic expansion, making it a therapeutic target for metastatic inhibition. Additionally, recombinant VEGFR3 serves as a critical tool in developing anti-angiogenic therapies and understanding congenital lymphatic disorders. Despite its therapeutic potential, challenges remain in achieving selective targeting to minimize off-effects on normal lymphatic functions. Ongoing research continues to explore its role in tissue repair and immune regulation, broadening its biomedical relevance.
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