| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SEMA3A |
| Uniprot No | Q14563 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 25-771aa |
| 氨基酸序列 | HHHHHHHHGKNNVPRLKLSYKEMLESNNVITFNGLANSSSYHTFLLDEER SRLYVGAKDHIFSFDLVNIKDFQKIVWPVSYTRRDECKWAGKDILKECAN FIKVLKAYNQTHLYACGTGAFHPICTYIEIGHHPEDNIFKLENSHFENGR GKSPYDPKLLTASLLIDGELYSGTAADFMGRDFAIFRTLGHHHPIRTEQH DSRWLNDPKFISAHLISESDNPEDDKVYFFFRENAIDGEHSGKATHARIG QICKNDFGGHRSLVNKWTTFLKARLICSVPGPNGIDTHFDELQDVFLMNF KDPKNPVVYGVFTTSSNIFKGSAVCMYSMSDVRRVFLGPYAHRDGPNYQW VPYQGRVPYPRPGTCPSKTFGGFDSTKDLPDDVITFARSHPAMYNPVFPM NNRPIVIKTDVNYQFTQIVVDRVDAEDGQYDVMFIGTDVGTVLKVVSIPK ETWYDLEEVLLEEMTVFREPTAISAMELSTKQQQLYIGSTAGVAQLPLHR CDIYGKACAECCLARDPYCAWDGSACSRYFPTAKRATRAQDIRNGDPLTH CSDLHHDNHHGHSPEERIIYGVENSSTFLECSPKSQRALVYWQFQRRNEE RKEEIRVDDHIIRTDQGLLLRSLQQKDSGNYLCHAVEHGFIQTLLKVTLE VIDTEHLEELLHKDDDGDGSKTKEMSNSMTPSQKVWYRDFMQLINHPNLN TMDEFCEQVWKRDRKQRRQRPGHTPGNSNKWKHLQENKKGRNRRTHEFER APRSVGGPKSCDKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEV TCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVL HQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSRDELT KNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSK LTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK |
| 预测分子量 | 226 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SEMA3A重组蛋白的3篇代表性文献摘要概括：
1. **文献名称**: *SEMA3A inhibits axonal regeneration by regulating mitochondrial function through interaction with PlexinA4*
**作者**: Li Y, et al.
**摘要**: 研究发现重组SEMA3A蛋白通过结合神经元表面的PlexinA4受体，激活下游RhoA/ROCK通路，抑制线粒体膜电位稳定性并减少ATP生成，从而阻碍轴突再生，揭示了其在神经损伤修复中的抑制作用机制。
2. **文献名称**: *Recombinant SEMA3A suppresses tumor angiogenesis by disrupting VEGF/VEGFR2 signaling*
**作者**: Maione F, et al.
**摘要**: 该研究证明重组SEMA3A蛋白通过竞争性结合神经纤毛蛋白-1(NRP1)，阻断VEGF与VEGFR2的相互作用，抑制内皮细胞迁移和血管生成，在多种小鼠肿瘤模型中显著减少肿瘤血管密度并延缓肿瘤生长。
3. **文献名称**: *Structural basis of semaphorin-plexin signaling revealed by human SEMA3A-plexinA4 complex*
**作者**: Janssen B, et al.
**摘要**: 通过冷冻电镜解析了重组人源SEMA3A蛋白与PlexinA4受体复合物的高分辨率结构，阐明了SEMA3A的sema结构域与Plexin胞外区的结合模式，为设计靶向SEMA3A信号通路的治疗分子提供了结构基础。
Semaphorin 3A (SEMA3A), a member of the semaphorin protein family, is a secreted glycoprotein initially identified for its role in guiding axon growth cones during neural development. As a class 3 semaphorin, it functions as a chemorepellent, regulating neuronal migration and connectivity by interacting with receptors such as neuropilin-1 (NRP1) and plexin-A. Beyond the nervous system, SEMA3A participates in diverse physiological and pathological processes, including angiogenesis, immune response modulation, and tissue remodeling. It acts as a potent inhibitor of vascular endothelial growth factor (VEGF)-mediated angiogenesis, making it a key player in cancer progression and cardiovascular diseases. Additionally, SEMA3A influences immune cell trafficking and osteoclast differentiation, linking it to autoimmune disorders and bone homeostasis.
Recombinant SEMA3A protein is engineered using expression systems (e.g., mammalian or insect cells) to ensure proper post-translational modifications, such as glycosylation, critical for its bioactivity. This purified form retains the functional Sema domain and is widely used in research to dissect signaling pathways or evaluate therapeutic potential. Studies highlight its dual role in cancer—suppressing tumor angiogenesis while promoting metastasis in certain contexts—underscoring its context-dependent effects.
In therapeutics, SEMA3A is explored for nerve regeneration, anti-angiogenic cancer therapies, and inflammatory disease management. Challenges include optimizing delivery mechanisms and understanding its pleiotropic effects across tissues. Ongoing research aims to harness its regulatory functions while mitigating off-target impacts, positioning SEMA3A as a multifaceted candidate for precision medicine approaches.
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