| 纯度 | >96%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RSPO3 |
| Uniprot No | Q9BXY4-1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-146aa |
| 氨基酸序列 | MHLRLISWLF IILNFMEYIG SQNASRGRRQ RRMHPNVSQG CQGGCATCSD YNGCLSCKPR LFFALERIGM KQIGVCLSSC PSGYYGTRYP DINKCTKCKA DCDTCFNKNF CTKCKSGFYL HLGKCLDNCP EGLEANNHTM ECVSIV |
| 预测分子量 | 15 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于RSPO3重组蛋白的3篇参考文献(基于公开数据模拟整理)：
1. **文献名称**：*R-spondin3 promotes stem cell recovery and epithelial regeneration in the colon*
**作者**：Kim KA, et al.
**摘要**：研究利用重组RSPO3蛋白，验证其通过激活Wnt/β-catenin信号通路促进结肠干细胞增殖和肠道上皮再生的作用，为肠道损伤修复提供潜在治疗策略。
2. **文献名称**：*Recombinant RSPO3 enhances bone regeneration by orchestrating osteoblast differentiation*
**作者**：Zhao J, et al.
**摘要**：通过体外实验和小鼠模型证明，重组RSPO3蛋白可通过调控Wnt信号通路，显著促进成骨细胞分化并加速骨缺损修复，提示其在骨组织工程中的应用潜力。
3. **文献名称**：*RSPO3-LGR4 axis drives chemoresistance in colorectal cancer via activation of Wnt signaling*
**作者**：Wang Y, et al.
**摘要**：研究发现肿瘤微环境中分泌的RSPO3重组蛋白与受体LGR4结合，激活Wnt通路并诱导结直肠癌细胞对化疗药物产生耐药性，为靶向干预提供新方向。
(注：以上文献信息为示例性模拟，实际引用请以真实论文数据为准。)
**Background of RSPO3 Recombinant Protein**
R-spondin 3 (RSPO3) is a secreted protein belonging to the R-spondin family, which comprises four members (RSPO1-4) known for their critical roles in regulating the Wnt/β-catenin signaling pathway. RSPO3 enhances Wnt signaling by binding to leucine-rich repeat-containing G-protein coupled receptors (LGR4-6) and neutralizing the transmembrane E3 ubiquitin ligases ZNRF3/RNF43. which otherwise promote Wnt receptor degradation. This interaction stabilizes Wnt receptors, amplifying Wnt signals essential for embryonic development, tissue homeostasis, and stem cell maintenance.
Structurally, RSPO3 contains two N-terminal furin-like cysteine-rich domains, a thrombospondin domain, and a C-terminal positively charged region. Its recombinant form is produced through genetic engineering in systems like mammalian cells (e.g., HEK293 or CHO cells) or *E. coli*, followed by purification via affinity chromatography. Recombinant RSPO3 retains biological activity, making it valuable for research and therapeutic exploration.
In research, RSPO3 recombinant protein is widely used to study Wnt-driven processes, such as intestinal stem cell proliferation, angiogenesis, and bone development. It also supports the growth of organoids—3D tissue models—by maintaining stem cell niches. Clinically, dysregulated RSPO3 is linked to cancers (e.g., colorectal, breast), fibrosis, and cardiovascular diseases, highlighting its potential as a therapeutic target. Inhibitors blocking RSPO3-Wnt interactions are under investigation, while recombinant RSPO3 itself may aid regenerative therapies for tissue repair.
Overall, RSPO3 recombinant protein serves as a vital tool for dissecting Wnt signaling mechanisms and developing strategies to modulate this pathway in disease contexts.
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