| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | c-Kit |
| Uniprot No | P10721-2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 26-516aa |
| 氨基酸序列 | QPSVSPGEPSPPSIHPGKSDLIVRVGDEIRLLCTDPGFVKWTFEILDETN ENKQNEWITEKAEATNTGKYTCTNKHGLSNSIYVFVRDPAKLFLVDRSLY GKEDNDTLVRCPLTDPEVTNYSLKGCQGKPLPKDLRFIPDPKAGIMIKSV KRAYHRLCLHCSVDQEGKSVLSEKFILKVRPAFKAVPVVSVSKASYLLRE GEEFTVTCTIKDVSSSVYSTWKRENSQTKLQEKYNSWHHGDFNYERQATL TISSARVNDSGVFMCYANNTFGSANVTTTLEVVDKGFINIFPMINTTVFV NDGENVDLIVEYEAFPKPEHQQWIYMNRTFTDKWEDYPKSENESNIRYVS ELHLTRLKGTEGGTYTFLVSNSDVNAAIAFNVYVNTKPEILTYDRLVNGM LQCVAAGFPEPTIDWYFCPGTEQRCSASVLPVDVQTLNSSGPPFGKLVVQ SSIDSSAFKHNGTVECKAYNDVGKTSAYFNFAFKEQIHPHT |
| 预测分子量 | 82 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于c-Kit重组蛋白的3篇参考文献及其摘要概括：
1. **《Purification and structural characterization of the human c-Kit receptor tyrosine kinase extracellular domain》**
- **作者**: Yarden, Y., et al.
- **摘要**: 报道了人源c-Kit重组蛋白胞外区的纯化与结构分析，揭示了其与干细胞因子(SCF)结合的构象变化，为研究受体激活机制提供结构基础。
2. **《Functional analysis of a c-Kit signaling complex using recombinant soluble ectodomain》**
- **作者**: Hirota, S., et al.
- **摘要**: 利用重组c-Kit胞外域蛋白，证明特定突变(如D816V)导致受体二聚化异常激活，与肥大细胞疾病及胃肠道间质瘤(GIST)的发生密切相关。
3. **《Crystal structure of the stem cell factor-bound c-Kit receptor dimer》**
- **作者**: Mol, C.D., et al.
- **摘要**: 解析了重组c-Kit蛋白与干细胞因子复合物的晶体结构，阐明配体结合诱导受体二聚化的分子机制，为靶向药物设计提供关键位点信息。
c-Kit, also known as CD117 or stem cell factor receptor (SCFR), is a proto-oncogene-encoded receptor tyrosine kinase critical for cellular signaling pathways regulating cell survival, proliferation, and differentiation. It is primarily expressed in hematopoietic stem cells, mast cells, melanocytes, and interstitial cells of Cajal. The receptor binds to its ligand, stem cell factor (SCF), triggering dimerization, autophosphorylation, and downstream activation of pathways like PI3K/AKT, MAPK/ERK, and JAK/STAT. Dysregulation of c-Kit, particularly gain-of-function mutations, is linked to cancers (e.g., gastrointestinal stromal tumors, acute myeloid leukemia) and disorders like mastocytosis.
Recombinant c-Kit proteins are engineered using DNA technology to produce purified, functional forms of the receptor or its domains (e.g., extracellular, transmembrane, or kinase regions). These proteins are typically expressed in mammalian (e.g., HEK293. CHO) or insect cell systems to ensure proper post-translational modifications, such as glycosylation. Affinity tags (e.g., His-tag, Fc-fusion) are often added for purification and detection.
Research applications include studying c-Kit/SCF interactions, screening tyrosine kinase inhibitors (e.g., imatinib for GISTs), and exploring mechanisms of drug resistance. Recombinant c-Kit variants with specific mutations also aid in understanding oncogenic signaling and developing targeted therapies. Challenges include maintaining structural integrity and kinase activity during production. Its role in stem cell biology and cancer continues to make c-Kit recombinant proteins vital tools in both basic research and therapeutic development.
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