| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/10-1/50 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Protein unc-50 homolog, Periodontal ligament-specific protein 22, PDLs22, Protein GMH1 homolog, hGMH1, Uncoordinated-like protein, UNC50, UNCL |
| Entrez GeneID | 25972 |
| WB Predicted band size | 30.4kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This UNC50 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 5-33 amino acids from the N-terminal region of human UNC50. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于UNC50 (N-term)抗体的3篇参考文献的简要信息(*注：以下为模拟内容，实际文献需根据具体数据库检索结果补充*)：
1. **文献名称**：*UNC50 regulates synaptic vesicle recycling in neuronal development*
**作者**：Smith A, et al.
**摘要**：该研究利用UNC50 (N-term)抗体进行免疫印迹和免疫荧光实验，证实UNC50蛋白在神经元突触小泡循环中的关键作用，并揭示其N端结构域与内体运输相关蛋白的相互作用。
2. **文献名称**：*A novel role of UNC50 in mRNA processing and export*
**作者**：Lee J, et al.
**摘要**：通过UNC50 (N-term)抗体的染色质免疫沉淀(ChIP)分析，研究发现UNC50参与RNA结合及核质转运，其N端结构域对mRNA加工复合体的组装至关重要。
3. **文献名称**：*UNC50 deficiency disrupts endoplasmic reticulum homeostasis in C. elegans*
**作者**：Chen R, et al.
**摘要**：利用UNC50 (N-term)抗体进行蛋白定位研究，揭示了UNC50在线虫内质网应激反应中的调控功能，并证明其N端缺失导致内质网结构异常。
(提示：实际文献需通过PubMed/Google Scholar检索“UNC50 antibody”或结合实验应用关键词筛选。)
The UNC50 (N-term) antibody is a tool for studying the UNC50 protein, which plays roles in intracellular trafficking and neuronal development. UNC50 (Uncoordinated 50) is a conserved eukaryotic protein implicated in regulating Golgi apparatus function and vesicle transport, particularly in maintaining the structure and activity of the Golgi network. It interacts with components of the COG (Conserved Oligomeric Golgi) complex, which is essential for glycosylation and membrane trafficking. UNC50 is also critical for synaptic vesicle recycling and neurotransmitter release, linking it to neuronal communication and nervous system development. Dysregulation of UNC50 has been associated with neurodevelopmental disorders and neurodegenerative conditions.
The UNC50 (N-term) antibody specifically targets the N-terminal region of the protein, enabling researchers to detect endogenous UNC50 expression via techniques like Western blotting, immunofluorescence, and immunohistochemistry. It is commonly produced in rabbits or mice, often as a polyclonal antibody, with validation in knockout models or siRNA-treated cells to confirm specificity. This antibody is valuable for investigating UNC50's localization, expression levels across tissues, and its role in cellular trafficking pathways or disease mechanisms. Its applications extend to studies on Golgi dysfunction, neuronal development, and pathologies like Alzheimer’s disease or cancer, where membrane trafficking anomalies are observed.
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