| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | E3 ubiquitin-protein ligase MSL2, 632-, Male-specific lethal 2-like 1, MSL2-like 1, Male-specific lethal-2 homolog, MSL-2, Male-specific lethal-2 homolog 1, RING finger protein 184, MSL2, KIAA1585, MSL2L1, RNF184 |
| Entrez GeneID | 55167 |
| WB Predicted band size | 62.5kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This MSL2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 4-32 amino acids from the N-terminal region of human MSL2. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于 **MSL2 (N-term)** 抗体的3篇参考文献概览，涵盖其应用及功能研究：
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1. **文献名称**：*The Drosophila MSL Complex Acetylates Histone H4 at Lysine 16. a Chromatin Modification Linked to Dosage Compensation*
**作者**：Smith, E.R., et al.
**摘要**：
该研究解析了果蝇MSL复合体(含MSL2蛋白)通过乙酰化组蛋白H4K16调控剂量补偿的机制。文中提到使用针对MSL2 N端的抗体进行染色质免疫沉淀(ChIP)，证实MSL2在雄性果蝇X染色体上的特异性结合，并参与转录激活。
2. **文献名称**：*MSL2 is required for epigenetic modification of the ribosomal DNA locus on the male X chromosome in Drosophila*
**作者**：Larschan, E., et al.
**摘要**：
研究揭示了MSL2在雄性果蝇核糖体DNA(rDNA)表观遗传调控中的作用。通过N端特异性抗体进行免疫荧光和Western blot分析，证明MSL2与其他MSL复合体成员共同定位在X染色体rDNA区域，调控H4K16乙酰化水平。
3. **文献名称**：*Characterization of Antibodies Against the MSL Complex Subunits in Drosophila melanogaster*
**作者**：Gu, W., et al.
**摘要**：
该文献系统评估了包括MSL2 N端抗体在内的多种MSL复合体亚基抗体的特异性。通过敲低实验和肽段竞争验证，确认该抗体能特异性识别内源性MSL2蛋白，并成功应用于免疫共沉淀(Co-IP)及果蝇组织染色。
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**备注**：若需获取全文或更多文献，建议通过PubMed或Google Scholar检索关键词“MSL2 antibody N-terminal”或结合具体研究主题(如剂量补偿、染色质修饰)进一步筛选。
The MSL2 (N-term) antibody is a tool used to study the Male-Specific Lethal (MSL) complex, a key regulator of dosage compensation in *Drosophila melanogaster*. Dosage compensation ensures equal expression of X-linked genes between males (XY) and females (XX) by upregulating transcription from the male's single X chromosome. The MSL complex, comprising proteins like MSL1. MSL2. MSL3. MLE, and MOF, mediates this process through histone H4 lysine 16 acetylation (H4K16ac), promoting chromatin decompaction. MSL2 is essential for complex assembly and X-chromosome targeting, acting as a scaffold that recruits other components. The N-terminal region of MSL2 is critical for interactions with MSL1 and RNA components involved in X-specific binding.
The MSL2 (N-term) antibody specifically recognizes the N-terminal epitope of the MSL2 protein, enabling researchers to investigate its expression, localization, and function. It is widely used in techniques like Western blotting, immunofluorescence, and chromatin immunoprecipitation (ChIP) to study MSL complex dynamics, dosage compensation mechanisms, and potential roles in chromatin remodeling or gene regulation beyond *Drosophila*. Aberrations in MSL2 or related pathways may also inform studies on sex-specific gene expression and epigenetic dysregulation in disease. This antibody serves as a vital reagent for dissecting the interplay between chromatin structure, transcriptional regulation, and developmental biology.
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