| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CTSH |
| Uniprot No | P09668 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-335aa |
| 氨基酸序列 | MWATLPLLCAGAWLLGVPVCGAAELCVNSLEKFHFKSWMSKHRKTYSTEEYHHRLQTFASNWRKINAHNNGNHTFKMALNQFSDMSFAEIKHKYLWSEPQNCSATKSNYLRGTGPYPPSVDWRKKGNFVSPVKNQGACGSCWTFSTTGALESAIAIATGKMLSLAEQQLVDCAQDFNNHGCQGGLPSQAFEYILYNKGIMGEDTYPYQGKDGYCKFQPGKAIGFVKDVANITIYDEEAMVEAVALYNPVSFAFEVTQDFMMYRTGIYSSTSCHKTPDKVNHAVLAVGYGEKNGIPYWIVKNSWGPQWGMNGYFLIERGKNMCGLAACASYPIPLV |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CTSH(组织蛋白酶H)重组蛋白的3篇代表性文献示例：
1. **文献名称**：*"Recombinant human procathepsin H is expressed as a stable enzyme in Escherichia coli and exhibits substrate specificity distinct from mature cathepsin H"*
**作者**：Smith J, et al.
**摘要**：该研究报道了通过大肠杆菌系统成功表达具有活性的重组人组织蛋白酶H前体蛋白，并发现其底物选择性与成熟酶存在差异，为研究其酶活调控机制提供了新思路。
2. **文献名称**：*"Structural and functional characterization of recombinant cathepsin H: Insights into its role in antigen processing"*
**作者**：Lee S, et al.
**摘要**：通过哺乳动物细胞系统表达重组CTSH，结合晶体结构分析和体外抗原降解实验，揭示了CTSH在抗原呈递通路中剪切特定肽键的关键作用。
3. **文献名称**：*"Development of a high-yield expression system for cathepsin H in Pichia pastoris and its application in cancer cell invasion assays"*
**作者**：Wang Y, et al.
**摘要**：研究利用毕赤酵母系统实现了CTSH重组蛋白的高效分泌表达，并证明其在体外可增强癌细胞的迁移能力，提示其在肿瘤转移中的潜在功能。
(注：以上文献为示例性内容，实际文献需通过PubMed或Google Scholar等平台检索确认。)
**Background of CTSH Recombinant Protein**
Cathepsin H (CTSH) is a lysosomal cysteine protease belonging to the papain-like protease family. It plays a critical role in intracellular protein degradation, antigen processing, and tissue remodeling. CTSH is synthesized as an inactive pro-enzyme, which undergoes proteolytic cleavage in lysosomes to become active. Unlike other cathepsins, CTSH exhibits both endopeptidase and aminopeptidase activities, enabling its involvement in diverse physiological processes, including apoptosis, immune response modulation, and extracellular matrix turnover. Dysregulation of CTSH has been linked to pathologies such as cancer, neurodegenerative disorders, and inflammatory diseases.
Recombinant CTSH protein is produced using biotechnological methods, often expressed in mammalian or insect cell systems to ensure proper post-translational modifications and enzymatic activity. This engineered protein retains the functional properties of native CTSH, including substrate specificity and pH-dependent activity, making it a valuable tool for biochemical and biomedical research. Applications include studying proteolytic pathways, screening protease inhibitors for drug development, and exploring its role in disease mechanisms.
Research highlights CTSH's dual role in cancer: promoting tumor invasion via extracellular matrix degradation while also exhibiting tumor-suppressive effects in certain contexts. Its involvement in neurodegenerative diseases like Alzheimer’s underscores its relevance in pathological protein aggregation. The availability of recombinant CTSH facilitates targeted studies to unravel its complex regulatory mechanisms and therapeutic potential.
In summary, CTSH recombinant protein serves as a crucial reagent for understanding protease biology and developing interventions for diseases linked to proteolytic imbalance.
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