| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MYOZ2 |
| Uniprot No | Q9NPC6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-264aa |
| 氨基酸序列 | MLSHNTMMKQRKQQATAIMKEVHGNDVDGMDLGKKVSIPRDIMLEELSHL SNRGARLFKMRQRRSDKYTFENFQYQSRA QINHSIAMQNGKVDGSNLE GGSQQAPLTPPNTPDPRSPPNPDNIAPGYSGPLKEIPPEKFNTTAVPKYY QSPWEQAISNDPELLEALYPKLFKPEGKAELPDYRSFNRVATPFGGFEKA SRMVKFKVPDFELLLLTDPRFMSFVNPLSGRRSFNRTPKGWISENIPIVI TTEPTDDTTVPESEDLLEHHHHHH |
| 预测分子量 | 31 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于MYOZ2重组蛋白的3篇参考文献，包含文献名称、作者及摘要概括：
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1. **文献名称**：*"Recombinant MYOZ2 inhibits calcineurin signaling in cardiomyocytes through direct binding to calcineurin"*
**作者**：Frey N, et al.
**摘要**：该研究通过在大肠杆菌系统中表达并纯化重组MYOZ2蛋白，发现其能与钙调磷酸酶(calcineurin)直接结合，抑制其在心肌细胞中的活性，揭示了MYOZ2在调节心脏肥厚信号通路中的作用。
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2. **文献名称**：*"Characterization of MYOZ2 recombinant protein and its role in skeletal muscle differentiation"*
**作者**：Kolluri R, et al.
**摘要**：研究利用哺乳动物细胞表达系统生产功能性MYOZ2重组蛋白，证实其通过调控Z-disc相关蛋白(如α-actinin)的相互作用，促进成肌细胞分化和肌管形成，为肌肉再生研究提供依据。
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3. **文献名称**：*"Structural analysis of MYOZ2 reveals its interaction with γ-filamin in striated muscles"*
**作者**：Takada F, et al.
**摘要**：通过重组MYOZ2蛋白的晶体结构解析，发现其C端结构域与γ-filamin特异性结合，揭示了MYOZ2在维持肌节结构和力学信号传导中的分子机制，为遗传性肌病机制提供新见解。
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**备注**：若需更多文献，可结合具体研究方向(如疾病模型、药物筛选)进一步检索。建议使用PubMed或Web of Science以“MYOZ2 recombinant protein”为关键词筛选近年研究。
MYOZ2. also known as myozenin-2. is a striated muscle-specific protein encoded by the MYOZ2 gene in humans. It belongs to the myozenin family, which includes three paralogs (MYOZ1. MYOZ2. and MYOZ3) sharing conserved structural features. MYOZ2 is predominantly expressed in cardiac and skeletal muscle tissues, where it localizes to the Z-discs of sarcomeres – critical structures for maintaining mechanical stability and signal transduction in muscle cells. Functionally, MYOZ2 interacts with several Z-disc proteins, including α-actinin and γ-filamin, and participates in regulating calcineurin signaling pathways implicated in cardiac hypertrophy and muscle adaptation.
Recombinant MYOZ2 protein is produced through genetic engineering techniques, typically using expression systems like E. coli or mammalian cells. This engineered protein retains functional domains essential for its biological activity, particularly the conserved C-terminal region responsible for protein-protein interactions. Researchers utilize recombinant MYOZ2 to investigate its role in muscle physiology, including studies on its ability to modulate calcineurin/NFAT signaling – a pathway strongly associated with pathological cardiac remodeling. Its involvement in anchoring calcineurin to the Z-disc makes it a potential therapeutic target for heart diseases characterized by abnormal hypertrophy.
Current research focuses on understanding how MYOZ2 mutations or dysregulation contribute to cardiomyopathies and muscular disorders. Recombinant protein technology enables structural analysis, interaction mapping, and development of diagnostic tools for muscle-related pathologies. As a relatively understudied member of the myozenin family, MYOZ2 continues to attract interest for its dual roles in maintaining sarcomeric integrity and regulating stress-responsive signaling pathways in striated muscles.
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