| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | STAT5B |
| Uniprot No | P51692 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-787aa |
| 氨基酸序列 | MAVWIQAQQLQGEALHQMQALYGQHFPIEVRHYLSQWIESQAWDSVDLDN PQENIKATQLLEGLVQELQKKAEHQVGEDGFLLKIKLGHYATQLQNTYDR CPMELVRCIRHILYNEQRLVREANNGSSPAGSLADAMSQKHLQINQTFEE LRLVTQDTENELKKLQQTQEYFIIQYQESLRIQAQFGPLAQLSPQERLSR ETALQQKQVSLEAWLQREAQTLQQYRVELAEKHQKTLQLLRKQQTIILDD ELIQWKRRQQLAGNGGPPEGSLDVLQSWCEKLAEIIWQNRQQIRRAEHLC QQLPIPGPVEEMLAEVNATITDIISALVTSTFIIEKQPPQVLKTQTKFAA TVRLLVGGKLNVHMNPPQVKATIISEQQAKSLLKNENTRNDYSGEILNNC CVMEYHQATGTLSAHFRNMSLKRIKRSDRRGAESVTEEKFTILFESQFSV GGNELVFQVKTLSLPVVVIVHGSQDNNATATVLWDNAFAEPGRVPFAVPD KVLWPQLCEALNMKFKAEVQSNRGLTKENLVFLAQKLFNNSSSHLEDYSG LSVSWSQFNRENLPGRNYTFWQWFDGVMEVLKKHLKPHWNDGAILGFVNK QQAHDLLINKPDGTFLLRFSDSEIGGITIAWKFDSQERMFWNLMPFTTRD FSIRSLADRLGDLNYLIYVFPDRPKDEVYSKYYTPVPCESATAKAVDGYV KPQIKQVVPEFVNASADAGGGSATYMDQAPSPAVCPQAHYNMYPQNPDSV LDTDGDFDLEDTMDVARRVEELLGRPMDSQWIPHAQS |
| 预测分子量 | 115 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于STAT5B重组蛋白的3篇代表性文献示例(注：文献信息为模拟示例，仅供参考)：
1. **文献名称**：*Structural insights into STAT5B activation by phosphorylation and ligand binding*
**作者**：Hennighausen L, et al.
**摘要**：通过重组STAT5B蛋白的晶体结构分析，揭示了其磷酸化后构象变化及DNA结合域的激活机制，为靶向STAT5B的药物设计提供结构基础。
2. **文献名称**：*Recombinant STAT5B expression in leukemia cells drives oncogenic signaling*
**作者**：Moriggl R, et al.
**摘要**：研究利用重组STAT5B蛋白在体外模型中验证其持续性激活可促进白血病细胞增殖，并阐明其与JAK2激酶的相互作用机制。
3. **文献名称**：*Functional characterization of STAT5B mutations in growth hormone resistance syndrome*
**作者**：Kazemi B, et al.
**摘要**：通过重组STAT5B突变体蛋白的功能实验，证明特定位点突变导致生长激素信号通路异常，与临床生长障碍表型直接相关。
4. **文献名称**：*Optimized prokaryotic expression and purification of bioactive STAT5B for in vitro assays*
**作者**：Reiter T, et al.
**摘要**：开发了一种高效的大肠杆菌表达系统，用于生产高纯度重组STAT5B蛋白，并验证其在体外磷酸化和DNA结合活性。
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(注：以上文献为示例性质，实际引用请通过PubMed或学术数据库检索真实发表论文。)
STAT5B, a member of the Signal Transducer and Activator of Transcription (STAT) protein family, is a critical intracellular signaling molecule involved in regulating gene expression in response to cytokines, growth factors, and hormones. It plays a central role in the JAK-STAT signaling pathway, mediating cellular processes such as proliferation, differentiation, survival, and immune responses. Structurally, STAT5B contains conserved domains including an N-terminal domain, coiled-coil domain, DNA-binding domain, SH2 domain, and a transactivation domain, which collectively enable its phosphorylation-dependent dimerization, nuclear translocation, and DNA-binding activity.
Recombinant STAT5B protein is engineered through genetic cloning and expression systems (e.g., bacterial, insect, or mammalian cells) to produce purified, functional protein for research and therapeutic applications. Its recombinant form retains the ability to be tyrosine-phosphorylated by upstream kinases (e.g., JAK kinases), facilitating dimerization and activation. Researchers utilize STAT5B recombinant protein to study signaling mechanisms, protein-protein/DNA interactions, and dysregulation in diseases such as cancers, immune disorders, and growth deficiencies. For instance, hyperactivation of STAT5B is linked to leukemia, while loss-of-function mutations are associated with growth hormone insensitivity syndromes.
The production of recombinant STAT5B often includes tags (e.g., GST, His-tag) for purification and detection. Its applications extend to in vitro kinase assays, structural studies, drug screening, and developing targeted therapies. As a tool, it helps dissect STAT5B's role in hematopoiesis, lactation, and metabolic regulation, offering insights into pathway crosstalk and therapeutic intervention strategies.
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