| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | STAT3 |
| Uniprot No | P40763 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-770aa |
| 氨基酸序列 | MAQWNQLQQLDTRYLEQLHQLYSDSFPMELRQFLAPWIESQDWAYAASKESHATLVFHNLLGEIDQQYSRFLQESNVLYQHNLRRIKQFLQSRYLEKPMEIARIVARCLWEESRLLQTAATAAQQGGQANHPTAAVVTEKQQMLEQHLQDVRKRVQDLEQKMKVVENLQDDFDFNYKTLKSQGDMQDLNGNNQSVTRQKMQQLEQMLTALDQMRRSIVSELAGLLSAMEYVQKTLTDEELADWKRRQQIACIGGPPNICLDRLENWITSLAESQLQTRQQIKKLEELQQKVSYKGDPIVQHRPMLEERIVELFRNLMKSAFVVERQPCMPMHPDRPLVIKTGVQFTTKVRLLVKFPELNYQLKIKVCIDKDSGDVAALRGSRKFNILGTNTKVMNMEESNNGSLSAEFKHLTLREQRCGNGGRANCDASLIVTEELHLITFETEVYHQGLKIDLETHSLPVVVISNICQMPNAWASILWYNMLTNNPKNVNFFTKPPIGTWDQVAEVLSWQFSSTTKRGLSIEQLTTLAEKLLGPGVNYSGCQITWAKFCKENMAGKGFSFWVWLDNIIDLVKKYILALWNEGYIMGFISKERERAILSTKPPGTFLLRFSESSKEGGVTFTWVEKDISGKTQIQSVEPYTKQQLNNMSFAEIIMGYKIMDATNILVSPLVYLYPDIPKEEAFGKYCRPESQEHPEADPGSAAPYLKTKFICVTPTTCSNTIDLPMSPRTLDSLMQFGNNGEGAEPSAGGQFESLTFDMELTSECATSPM |
| 预测分子量 | 135.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于STAT3重组蛋白的3篇代表性文献，简要概括如下：
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1. **文献名称**：*Structure of the STAT3 transcription factor homodimer bound to DNA*
**作者**：Becker, S., Groner, B., & Müller, C.W. (1998)
**摘要**：该研究通过X射线晶体学解析了STAT3重组蛋白与DNA结合的复合物结构，揭示了STAT3二聚体的构象及其与靶DNA结合的分子机制，为理解STAT3的转录调控功能提供了结构基础。
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2. **文献名称**：*STAT3 activation by Src induces specific gene regulation and cellular transformation*
**作者**：Bromberg, J.F., Horvath, C.M., Besser, D., et al. (1998)
**摘要**：文章阐明了STAT3重组蛋白在Src激酶介导下的磷酸化激活过程，并证明其异常活化可促进细胞转化和肿瘤发生，揭示了STAT3在致癌信号通路中的关键作用。
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3. **文献名称**：*Selective chemical probe inhibitor of STAT3 identified through structure-based virtual screening*
**作者**：Siddiquee, K., Zhang, S., Guida, W.C., et al. (2007)
**摘要**：研究利用重组STAT3蛋白进行高通量筛选，发现了一种特异性小分子抑制剂(STA-21)，通过阻断STAT3的DNA结合域抑制其活性，为靶向STAT3的抗癌药物开发提供了实验依据。
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这些文献涵盖了STAT3重组蛋白的结构解析、功能机制及治疗应用，均为该领域的经典研究。如需具体期刊信息或DOI，可进一步补充。
**Background of STAT3 Recombinant Protein**
STAT3 (Signal Transducer and Activator of Transcription 3) is a critical transcription factor belonging to the STAT family, which mediates cellular responses to cytokines, growth factors, and environmental stimuli. Structurally, STAT3 contains conserved domains including an N-terminal domain, a coiled-coil domain, a DNA-binding domain, a SH2 domain crucial for phosphotyrosine-mediated dimerization, and a C-terminal transcriptional activation domain. Upon activation by receptor-associated kinases (e.g., JAKs) or growth factor receptors (e.g., EGFR), STAT3 undergoes tyrosine phosphorylation at residue Y705. leading to dimerization, nuclear translocation, and regulation of target genes involved in cell proliferation, survival, differentiation, and immune responses.
Dysregulated STAT3 signaling is implicated in numerous pathologies, particularly cancer, where constitutive activation promotes tumor growth, metastasis, and therapy resistance. It also plays dual roles in inflammation, contributing to both pro-inflammatory and anti-inflammatory processes depending on cellular context. These features make STAT3 a key therapeutic target, driving demand for tools to study its function.
Recombinant STAT3 proteins are engineered using expression systems (e.g., *E. coli*, mammalian cells*) to produce purified, bioactive forms of STAT3. often tagged with markers like His or GST for purification and detection. These proteins enable *in vitro* studies on phosphorylation kinetics, dimerization, DNA-binding activity, and interactions with inhibitors or partners. They are widely used in drug discovery to screen small-molecule inhibitors, in mechanistic studies to dissect signaling pathways, and as antigens for antibody development. Additionally, mutant variants of recombinant STAT3 help elucidate structure-function relationships or disease-associated mutations.
Overall, STAT3 recombinant proteins serve as indispensable tools for advancing research in oncology, immunology, and inflammatory diseases, bridging molecular insights to therapeutic innovation.
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