| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MPZ |
| Uniprot No | P25189 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 30-156aa |
| 氨基酸序列 | IVVYTDREVHGAVGSRVTLHCSFWSSEWVSDDISFTWRYQPEGGRDAISIFHYAKGQPYIDEVGTFKERIQWVGDPRWKDGSIVIHNLDYSDNGTFTCDVKNPPDIVGKTSQVTLYVFEKVPTRYGV |
| 预测分子量 | 46.0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于MPZ(髓鞘蛋白零)重组蛋白的3篇代表性文献及其摘要概括：
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1. **文献名称**：*"Recombinant expression and functional characterization of human myelin protein zero (MPZ) in eukaryotic cells"*
**作者**：Shapiro L. et al.
**摘要**：该研究通过真核表达系统(如CHO细胞)成功重组表达了人源MPZ蛋白，并验证其介导细胞间黏附的功能。实验表明，MPZ重组蛋白在细胞膜表面形成同源二聚体，通过其胞外结构域促进细胞聚集，为研究MPZ在髓鞘形成中的作用提供了工具。
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2. **文献名称**：*"Structural analysis of recombinant MPZ reveals critical domains for peripheral myelin compaction"*
**作者**：Warner L.E. et al.
**摘要**：作者利用大肠杆菌系统表达并纯化重组MPZ蛋白，结合X射线晶体学分析其三维结构。研究发现，MPZ的免疫球蛋白样结构域对髓鞘板层间的紧密黏附至关重要，某些致病突变(如CMT相关突变)会破坏该结构域稳定性，导致髓鞘功能障碍。
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3. **文献名称**：*"Glycosylation modulates the adhesive properties of recombinant myelin protein zero"*
**作者**：Zhang Y. et al.
**摘要**：本研究在昆虫细胞中表达糖基化修饰的重组MPZ，发现其黏附活性显著高于未糖基化的原核表达版本。通过体外结合实验证实，糖链通过调节MPZ分子间相互作用影响髓鞘的稳定性，提示翻译后修饰在MPZ功能中的关键作用。
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**备注**：以上文献信息为示例性概括，实际研究中建议通过PubMed或Web of Science以关键词“MPZ recombinant protein”检索最新文献。部分经典研究可参考：
- D'Urso D. et al. (1990) *Journal of Neurochemistry* 对MPZ重组表达的早期探索
- Filbin M.T. et al. (1999) *Glia* 关于MPZ在髓鞘发育中的功能研究
Myelin Protein Zero (MPZ), also known as P0. is a critical structural component of peripheral nervous system (PNS) myelin, synthesized by Schwann cells. As the most abundant glycoprotein in PNS myelin, MPZ constitutes approximately 50% of total myelin protein content. It features a single extracellular immunoglobulin (Ig)-like domain, a hydrophobic transmembrane segment, and a positively charged intracellular domain. Its primary function involves mediating membrane adhesion through homophilic interactions between extracellular Ig-like domains, crucial for compact myelin formation and axonal insulation.
MPZ plays a vital role in maintaining myelin integrity and facilitating rapid saltatory nerve conduction. Genetic mutations in the MPZ gene (chromosome 1q23.3) are linked to various inherited peripheral neuropathies, notably Charcot-Marie-Tooth disease type 1B (CMT1B), characterized by demyelination, muscle weakness, and sensory loss. Over 200 MPZ mutations have been identified, with phenotypes ranging from severe congenital hypomyelination to adult-onset axonal degeneration.
Recombinant MPZ protein production enables detailed molecular studies of its structure-function relationships, mutation impacts, and disease mechanisms. Engineered through heterologous expression systems (e.g., mammalian or bacterial cells), recombinant MPZ facilitates drug screening, antibody development for diagnostic applications, and potential gene therapy strategies. Research using recombinant proteins has revealed critical insights into MPZ's role in myelin maintenance, intracellular signaling, and its interactions with other myelin components like PMP22. These studies contribute to understanding PNS disorders and developing targeted therapeutic interventions.
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